Altered Lung Heat Shock Protein-70 Expression and Severity of Sepsis-Induced Acute Lung Injury in a Chronic Kidney Disease Rat Model
Abstract
:1. Introduction
1.1. Sepsis-Induced Acute Lung Injury
1.2. Role of Heat Shock Protein in Acute Lung Injury
1.3. Chronic Kidney Disease (CKD) and Lung Disorder
1.4. Objective of the Study
2. Results
2.1. Clinical Parameters
2.2. Severity of Acute Lung Injury
2.3. Lung HSP-70 Expression
2.4. Serum IL-6 Immunoassay
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Sepsis and Chronic Kidney Disease Model
4.3. Blood and Urine Test
4.4. Histologic Analysis and Immunohistochemistry
4.5. Western Blotting
4.6. Interleukin-6 Immunoassay
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Control | Control with Sepsis at 72 h | CKD | CKD with Sepsis at 72 h | |
---|---|---|---|---|
Body weight (g) | 520.0 ± 21.3 | 451.2 ± 51.0 * | 425.5 ± 52.4 ** | 415.2 ± 33.3 ** |
Urine volume for 24 h (mL) | 11.4 ± 1.3 | 11.6 ± 5.2 | 30.5 ± 5.2 **, ΥΥ | 23.4 ± 8.8 **, Υ |
Urine pH | 7.43 ± 0.55 | 9.16 ± 0.12 ** | 7.56 ± 0.28 ΥΥ | 8.67 ± 0.27 **, ΥΥ, ¥¥ |
Serum Cr (mg/dL) | 0.40 ± 0.03 | 0.54 ± 0.28 | 0.90 ± 0.26 ** | 0.90 ± 0.17 **, Υ |
BUN (mg/dL) | 22.9 ± 3.9 | 25.85 ± 10.7 | 53.6 ± 14.4 **, Υ | 37.4 ± 4.5 **, ¥ |
Serum albumin (mg/dL) | 2.3 ± 0.2 | 1.78 ± 02 ** | 2.1 ± 0.2 Υ | 1.9 ± 0.2 * |
ClCr (mL/min/100 g) | 0.37 ± 0.06 | 0.32 ± 0.20 | 0.15 ± 0.04 ** | 0.21 ± 0.03 **, ¥¥ |
Plasma CRP | 0.02 ± 0.00 | 0.02 ± 0.00 | 0.02 ± 0.00 | 0.02 ± 0.00 |
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Cho, J.-Y.; Kim, S.-J.; Woo, C.-G.; Kwon, S.-K.; Choe, K.-H.; Kim, E.-G.; Shin, Y.-M. Altered Lung Heat Shock Protein-70 Expression and Severity of Sepsis-Induced Acute Lung Injury in a Chronic Kidney Disease Rat Model. Int. J. Mol. Sci. 2023, 24, 5641. https://doi.org/10.3390/ijms24065641
Cho J-Y, Kim S-J, Woo C-G, Kwon S-K, Choe K-H, Kim E-G, Shin Y-M. Altered Lung Heat Shock Protein-70 Expression and Severity of Sepsis-Induced Acute Lung Injury in a Chronic Kidney Disease Rat Model. International Journal of Molecular Sciences. 2023; 24(6):5641. https://doi.org/10.3390/ijms24065641
Chicago/Turabian StyleCho, Jun-Yeun, Seung-Jung Kim, Chang-Gok Woo, Soon-Kil Kwon, Kang-Hyeon Choe, Eung-Gook Kim, and Yoon-Mi Shin. 2023. "Altered Lung Heat Shock Protein-70 Expression and Severity of Sepsis-Induced Acute Lung Injury in a Chronic Kidney Disease Rat Model" International Journal of Molecular Sciences 24, no. 6: 5641. https://doi.org/10.3390/ijms24065641