Strain-Specific Interactions between the Viral Capsid Proteins VP4, VP7 and VP6 Influence Rescue of Rotavirus Reassortants by Reverse Genetics
Abstract
:1. Introduction
2. Results
2.1. Generation of VP7-Wa- and VP6-Wa-Encoding Plasmids
2.2. Generation of Mono-, Double-, and Triple-Reassortants
2.3. Transmission Electron Microscopy (TEM) Analyses
2.4. Replication Kinetics
2.5. Untranslated Region (UTR) Sequence Analyses
2.6. Protein Interface Analyses
3. Discussion
4. Materials and Methods
4.1. Cell Lines and Viruses
4.2. Plasmids
4.3. Plasmid-Based RG System
4.4. Passaging of Reassortant Virus
4.5. qRT-PCR and RT-PCR
4.6. Replication Kinetics
4.7. Plaque Assays
4.8. Transmission Electron Microscope (TEM) Analyses
4.9. Sequence Analyses and Protein Structure Visualization
4.10. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strain | Genotype | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
VP7 | VP4 | VP6 | VP1 | VP2 | VP3 | NSP1 | NSP2 | NSP3 | NSP4 | NSP5 | |
SA11 | G3 | P[2] | I2 | R2 | C5 | M5 | A5 | N5 | T5 | E2 | H5 |
Wa | G1 | P[8] | I1 | R1 | C1 | M1 | A1 | N1 | T1 | E1 | H1 |
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Valusenko-Mehrkens, R.; Gadicherla, A.K.; Johne, R.; Falkenhagen, A. Strain-Specific Interactions between the Viral Capsid Proteins VP4, VP7 and VP6 Influence Rescue of Rotavirus Reassortants by Reverse Genetics. Int. J. Mol. Sci. 2023, 24, 5670. https://doi.org/10.3390/ijms24065670
Valusenko-Mehrkens R, Gadicherla AK, Johne R, Falkenhagen A. Strain-Specific Interactions between the Viral Capsid Proteins VP4, VP7 and VP6 Influence Rescue of Rotavirus Reassortants by Reverse Genetics. International Journal of Molecular Sciences. 2023; 24(6):5670. https://doi.org/10.3390/ijms24065670
Chicago/Turabian StyleValusenko-Mehrkens, Roman, Ashish K. Gadicherla, Reimar Johne, and Alexander Falkenhagen. 2023. "Strain-Specific Interactions between the Viral Capsid Proteins VP4, VP7 and VP6 Influence Rescue of Rotavirus Reassortants by Reverse Genetics" International Journal of Molecular Sciences 24, no. 6: 5670. https://doi.org/10.3390/ijms24065670