Actin Depolymerization Factor ADF1 Regulated by MYB30 Plays an Important Role in Plant Thermal Adaptation
Abstract
:1. Introduction
2. Results
2.1. The Expression of AtADF1 Is Down-Regulated under High Temperature
2.2. AtADF1 Is a Negative Regulator of Plant Growth and Actin Filament Stability under High Temperature
2.3. The Expression of AtADF1 Is Positively Regulated by AtMYB30 under High Temperature and AtADF1 Is a Direct Target Gene of AtMYB30
2.4. Sequence Analysis of Chinese Cabbage ADF1 (BrADF1)
2.5. BrADF1 Inhibits Plant Growth and Actin Filaments Stability under High Temperature
3. Discussion
3.1. AtADF1, Mediated by AtMYB30, Plays a Negative Role in Plant Thermal Adaptation by Affecting Actin Filament Organization
3.2. BrADF1 Plays a Similar and Important Role to AtADF1 in Plant Thermal Adaptation
4. Materials and Methods
4.1. Plant Materials, Growth Conditions, and Heat Treatments
4.2. Gene Expression and GUS Activity Analysis
4.3. Chromatin Immunoprecipitation-Quantitative PCR (ChIP-qPCR) Assay
4.4. Electrophoretic Mobility Shift Assays (EMSA)
4.5. Sequence Analysis of BrADF1
4.6. Plasmid Construction and Plant Transformation
4.7. Visualization and Analysis of Actin Filament Architecture and the Subcellular Localization of BrADF1
4.8. Western Blot Assays
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wang, L.; Cheng, J.; Bi, S.; Wang, J.; Cheng, X.; Liu, S.; Gao, Y.; Lan, Q.; Shi, X.; Wang, Y.; et al. Actin Depolymerization Factor ADF1 Regulated by MYB30 Plays an Important Role in Plant Thermal Adaptation. Int. J. Mol. Sci. 2023, 24, 5675. https://doi.org/10.3390/ijms24065675
Wang L, Cheng J, Bi S, Wang J, Cheng X, Liu S, Gao Y, Lan Q, Shi X, Wang Y, et al. Actin Depolymerization Factor ADF1 Regulated by MYB30 Plays an Important Role in Plant Thermal Adaptation. International Journal of Molecular Sciences. 2023; 24(6):5675. https://doi.org/10.3390/ijms24065675
Chicago/Turabian StyleWang, Lu, Jianing Cheng, Shuangtian Bi, Jinshu Wang, Xin Cheng, Shihang Liu, Yue Gao, Qingkuo Lan, Xiaowei Shi, Yong Wang, and et al. 2023. "Actin Depolymerization Factor ADF1 Regulated by MYB30 Plays an Important Role in Plant Thermal Adaptation" International Journal of Molecular Sciences 24, no. 6: 5675. https://doi.org/10.3390/ijms24065675