Impaired Integrated Stress Response and Mitochondrial Integrity Modulate Genotoxic Stress Impact and Lower the Threshold for Immune Signalling
Abstract
:1. Introduction
2. Results and Discussion
2.1. Mitochondrial Homeostasis Is Influenced by Mitochondrial Quality Control and ISR Integrity
2.2. Sensitivity to Genotoxic Stressors
2.3. Signalling Mechanisms Revealed by RNA Sequencing
3. Materials and Methods
3.1. Cell Culture
3.2. Genotoxic Treatment
3.3. Bystander Effect
3.4. Cell Viability
3.5. ATP Level Measurement
3.6. Protein Level Measurement (Bradford Assay)
3.7. Gene Expression Analysis
3.8. High Resolution Respirometry
3.9. Micronuclei (MN) Analysis
3.10. Statistical Analyses
3.11. RNA Sequencing Analysis
3.12. Differential Expression Analysis and Pathway Enrichment Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Temelie, M.; Talpur, R.; Dominguez-Prieto, M.; Dantas Silva, A.; Cenusa, C.; Craciun, L.; Savu, D.I.; Moisoi, N. Impaired Integrated Stress Response and Mitochondrial Integrity Modulate Genotoxic Stress Impact and Lower the Threshold for Immune Signalling. Int. J. Mol. Sci. 2023, 24, 5891. https://doi.org/10.3390/ijms24065891
Temelie M, Talpur R, Dominguez-Prieto M, Dantas Silva A, Cenusa C, Craciun L, Savu DI, Moisoi N. Impaired Integrated Stress Response and Mitochondrial Integrity Modulate Genotoxic Stress Impact and Lower the Threshold for Immune Signalling. International Journal of Molecular Sciences. 2023; 24(6):5891. https://doi.org/10.3390/ijms24065891
Chicago/Turabian StyleTemelie, Mihaela, Rubab Talpur, Marta Dominguez-Prieto, Ayanda Dantas Silva, Constantin Cenusa, Liviu Craciun, Diana Iulia Savu, and Nicoleta Moisoi. 2023. "Impaired Integrated Stress Response and Mitochondrial Integrity Modulate Genotoxic Stress Impact and Lower the Threshold for Immune Signalling" International Journal of Molecular Sciences 24, no. 6: 5891. https://doi.org/10.3390/ijms24065891