Kisspeptin-10 Mitigates α-Synuclein-Mediated Mitochondrial Apoptosis in SH-SY5Y-Derived Neurons via a Kisspeptin Receptor-Independent Manner
Abstract
:1. Introduction
2. Results
2.1. KP-10 Attenuates α-Syn-Mediated Apoptotic Death in Cholinergic-like Neurons via a GPR54-Independent Manner
2.2. KP-10 Rescues α-Syn-Induced Mitochondrial Depolarization in Cholinergic-like Neurons through a GPR54 Dispensable Mechanism
2.3. KP-10 Diminishes α-Syn and ChAT Immunofluorescence Intensity in Cholinergic-like Neurons Overexpressing Human Wild-Type or E46K Mutant α-Syn
3. Discussion
4. Materials and Methods
4.1. Kisspeptin Peptides
4.2. α-Synuclein Plasmids
4.3. Cell Culture
4.4. Differentiation into Cholinergic-like Neurons
4.5. Transient Transfections
4.6. Quantification of Apoptosis by Annexin-V Labeling
4.7. Mitochondrial Membrane Potential
4.8. Double Label Immunofluorescence
4.9. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Simon, C.; Soga, T.; Parhar, I. Kisspeptin-10 Mitigates α-Synuclein-Mediated Mitochondrial Apoptosis in SH-SY5Y-Derived Neurons via a Kisspeptin Receptor-Independent Manner. Int. J. Mol. Sci. 2023, 24, 6056. https://doi.org/10.3390/ijms24076056
Simon C, Soga T, Parhar I. Kisspeptin-10 Mitigates α-Synuclein-Mediated Mitochondrial Apoptosis in SH-SY5Y-Derived Neurons via a Kisspeptin Receptor-Independent Manner. International Journal of Molecular Sciences. 2023; 24(7):6056. https://doi.org/10.3390/ijms24076056
Chicago/Turabian StyleSimon, Christopher, Tomoko Soga, and Ishwar Parhar. 2023. "Kisspeptin-10 Mitigates α-Synuclein-Mediated Mitochondrial Apoptosis in SH-SY5Y-Derived Neurons via a Kisspeptin Receptor-Independent Manner" International Journal of Molecular Sciences 24, no. 7: 6056. https://doi.org/10.3390/ijms24076056