Wds-Mediated H3K4me3 Modification Regulates Lipid Synthesis and Transport in Drosophila
Abstract
:1. Introduction
2. Results
2.1. Disrupting Wds-Mezdiated H3K4me3 Modification in the Drosophila Fat Body Reduced Lipid Content
2.2. Fat Body-Specific Wds Knockdown Impaired the Transcription of Lipogenic Genes by Reducing H3K4me3 Deposition within Their Promoters
2.3. Wds-Mediated H3K4me3 Modification Regulates Lipid Transport from Intestine by Modulating the Expression of the Lpp Gene in the Fat Body
2.4. Transcription Factor Hsf Promotes Lipid Synthesis and Transport by Interacting with Wds to Modulate H3K4me3 Modification
2.5. HFD Condition Promotes Wds-Mediated H3K4me3 in the Drosophila Fat Body
3. Discussion
4. Materials and Methods
4.1. Drosophila Cultivation and Stocks
4.2. Immunostaining
4.3. RNA-seq
4.4. Cell Culture and Transfection
4.5. Co-Immunoprecipitation (Co-IP)
4.6. Western Blotting
4.7. Chromatin Immunoprecipitation Sequencing (ChIP-seq)
4.8. RNA Extraction and Real-Time Quantitative PCR (RT-qPCR)
4.9. TG Measurement
4.10. Databases
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhao, T.; Wang, M.; Li, Z.; Li, H.; Yuan, D.; Zhang, X.; Guo, M.; Qian, W.; Cheng, D. Wds-Mediated H3K4me3 Modification Regulates Lipid Synthesis and Transport in Drosophila. Int. J. Mol. Sci. 2023, 24, 6125. https://doi.org/10.3390/ijms24076125
Zhao T, Wang M, Li Z, Li H, Yuan D, Zhang X, Guo M, Qian W, Cheng D. Wds-Mediated H3K4me3 Modification Regulates Lipid Synthesis and Transport in Drosophila. International Journal of Molecular Sciences. 2023; 24(7):6125. https://doi.org/10.3390/ijms24076125
Chicago/Turabian StyleZhao, Tujing, Min Wang, Zheng Li, Hao Li, Dongqin Yuan, Xing Zhang, Mengge Guo, Wenliang Qian, and Daojun Cheng. 2023. "Wds-Mediated H3K4me3 Modification Regulates Lipid Synthesis and Transport in Drosophila" International Journal of Molecular Sciences 24, no. 7: 6125. https://doi.org/10.3390/ijms24076125