ZNF750: A Novel Prognostic Biomarker in Metastatic Prostate Cancer
Abstract
:1. Introduction
2. Results
2.1. Clinical Features of the Patient Cohort
2.2. ZNF750 Expression Is Reduced in Prostate Cancer
2.3. Loss of ZNF750 Nuclear Expression Predicts Risk of Metastatic Prostate Cancer
2.4. Low Levels of ZNF750 Are Associated with a Worse Prognosis
3. Discussion
4. Materials and Methods
4.1. Case Selection
4.2. Cell Culture
4.3. Immunohistochemical Analysis
4.4. RNA Isolation and Quantitative Real-Time PCR
4.5. Confocal Microscopy
4.6. Promoter Methylation
4.7. Bioinformatics Analysis and Mutational Analysis
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Grade Group/Gleason Score | Cases No. (%) | Metastasis No. (%) |
---|---|---|
1/6 (3 + 3) | 4 (9.3%) | 1 (5.9%) |
2/7 (3 + 4) | 13 (30.2%) | 2 (11.8%) |
3/7 (4 + 3) | 7 (16.3%) | 3 (17.6%) |
4/8 (4 + 4) | 17 (39.5%) | 9 (52.9%) |
5/9 (4 + 5) | 2 (4.7%) | 2 (11.8%) |
Tot. | 43 (100%) | 17 (100%) |
(A) | |||
ZNF750 Cytoplasmatic Staining | |||
Negative | Positive | p | |
Benign Hyperplasia | 0 (0%) | 23 (100%) | 0.001 |
Acinar Carcinoma | 28 (82.4%) | 6 (17.6%) | |
Gleason Score | 0.33 | ||
6 | 2 (5.8%) | 2 (22.2%) | |
7 | 17 (50.0%) | 3 (33.3%) | |
8 | 13 (35.4%) | 4 (44.5%) | |
9 | 2 (8.8%) | 0 (0%) | |
Grade Group | 0.37 | ||
1 | 2 (5.9%) | 2 (22.2%) | |
2 | 12 (35.3%) | 1 (11.1%) | |
3 | 5 (14.7%) | 2 (22.2%) | |
4/5 | 15 (44.1%) | 4 (44.5%) | |
Metastasis | 0.006 | ||
with | 17 (100%) | 0 (0%) | |
w/o | 17 (65.4%) | 9 (34.6%) | |
(B) | |||
ZNF750 Nuclear Staining | |||
Negative | Positive | p | |
Benign Hyperplasia | 0 (0%) | 23 (100%) | 0.001 |
Acinar Carcinoma | 11 (34.4%) | 21 (65.6%) | |
Gleason Score | 0.16 | ||
6 | 0 (0%) | 4 (12.9%) | |
7 | 4 (33.3%) | 16 (51.6%) | |
8 | 6 (50.0%) | 10 (32.3%) | |
9 | 2 (16.7%) | 1 (3.2%) | |
Grade Group | 0.09 | ||
1 | 0 (0%) | 4 (12.9%) | |
2 | 3 (25.0%) | 10 (32.3%) | |
3 | 1 (8.3%) | 6 (19.3%) | |
4/5 | 8 (66.7%) | 11 (35.5%) | |
Metastasis | 0.01 | ||
with | 10 (58.8%) | 7 (41.2%) | |
w/o | 2 (7.7%) | 24 (92.3%) |
p | Odds Ratio (EXPB) | 95% C.I. | |
---|---|---|---|
Grade Group | 0.10 | 1.89 | 0.88–4.10 |
Absence of ZNF750 nuclear positivity | 0.005 | 13.40 | 2.22–80.81 |
Gleason Score | 0.33 | 1.70 | 0.58–4.98 |
Absence of ZNF750 nuclear positivity | 0.004 | 13.71 | 2.32–81.02 |
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Share and Cite
Montanaro, M.; Agostini, M.; Anemona, L.; Bonanno, E.; Servadei, F.; Finazzi Agrò, E.; Asimakopoulos, A.D.; Ganini, C.; Cipriani, C.; Signoretti, M.; et al. ZNF750: A Novel Prognostic Biomarker in Metastatic Prostate Cancer. Int. J. Mol. Sci. 2023, 24, 6519. https://doi.org/10.3390/ijms24076519
Montanaro M, Agostini M, Anemona L, Bonanno E, Servadei F, Finazzi Agrò E, Asimakopoulos AD, Ganini C, Cipriani C, Signoretti M, et al. ZNF750: A Novel Prognostic Biomarker in Metastatic Prostate Cancer. International Journal of Molecular Sciences. 2023; 24(7):6519. https://doi.org/10.3390/ijms24076519
Chicago/Turabian StyleMontanaro, Manuela, Massimiliano Agostini, Lucia Anemona, Elena Bonanno, Francesca Servadei, Enrico Finazzi Agrò, Anastasios D. Asimakopoulos, Carlo Ganini, Chiara Cipriani, Marta Signoretti, and et al. 2023. "ZNF750: A Novel Prognostic Biomarker in Metastatic Prostate Cancer" International Journal of Molecular Sciences 24, no. 7: 6519. https://doi.org/10.3390/ijms24076519