Molecular Physiological Evidence for the Role of Na+-Cl− Co-Transporter in Branchial Na+ Uptake in Freshwater Teleosts
Abstract
:1. Introduction
2. Results
2.1. Branchial NCC Ionocytes Are a Specific Subtype of Ionocytes
2.2. Low-Na+ FW Increased the Branchial Protein Expression of Ncc2 but Did Not Affect Its mRNA Expression
2.3. Low-Na+ FW Increased the Number of NCC Ionocytes and the Ncc2 Protein Expression of Individual NCC Ionocytes in the Gills
2.4. Low-Na+ FW Elevated Branchial Na+ Uptake
2.5. Cl−-Free FW Decreased Na+ Uptake in the Gills Acclimated to Low-Na+ FW
2.6. Ncc Inhibitor Impaired Na+ Uptake in the Gills Acclimated to Low-Na+ FW
2.7. Nhe Inhibitor Impaired Na+ Uptake in the Gills Acclimated to Low-Na+ FW
3. Discussion
4. Materials and Methods
4.1. Experimental Animals
4.2. Single-Cell RNA Sequencing (scRNA-Seq)
4.3. Low-Na+ Acclimation Experiment
4.4. Preparation of Complementary DNA
4.5. Quantitative Real-time Polymerase Chain Reaction (qRT-PCR)
4.6. Immunofluorescence (IF)
4.7. Western Blot
4.8. Scanning Ion-Selective Electrode Technique (SIET)
4.9. Pharmacological Treatments
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
Asic | Acid-sensing ion channel |
CD | Collecting duct |
DCT | Distal convoluted tubule |
dpf | Day(s) post-fertilization |
Ecac | Epithelial Ca2+ channel |
EIPA | 5-(N-ethyl-N-isopropyl)-amiloride |
Enac | Epithelial Na+ channel |
FW | Freshwater |
GFR | Glomerular filtration rate |
HR | VHa-rich |
IF | Immunofluorescence |
KO | Knockout |
NaR | Nka-rich |
Ncc | Na+-Cl− co-transporter |
Nhe | Na+/H+ exchanger |
Nka | Na+/K+ ATPase |
PCT | Proximal convoluted tubule |
qRT-PCR | Quantitative real-time polymerase chain reaction |
scRNA-Seq | Single-cell RNA sequencing |
SIET | Scanning ion-selective electrode technique |
SW | Seawater |
VHa | Vascular-type H+ ATPase |
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Shih, S.-W.; Yan, J.-J.; Lu, S.-W.; Chuang, Y.-T.; Lin, H.-W.; Chou, M.-Y.; Hwang, P.-P. Molecular Physiological Evidence for the Role of Na+-Cl− Co-Transporter in Branchial Na+ Uptake in Freshwater Teleosts. Int. J. Mol. Sci. 2023, 24, 6597. https://doi.org/10.3390/ijms24076597
Shih S-W, Yan J-J, Lu S-W, Chuang Y-T, Lin H-W, Chou M-Y, Hwang P-P. Molecular Physiological Evidence for the Role of Na+-Cl− Co-Transporter in Branchial Na+ Uptake in Freshwater Teleosts. International Journal of Molecular Sciences. 2023; 24(7):6597. https://doi.org/10.3390/ijms24076597
Chicago/Turabian StyleShih, Shang-Wu, Jia-Jiun Yan, Shao-Wei Lu, Ya-Ting Chuang, How-Wei Lin, Ming-Yi Chou, and Pung-Pung Hwang. 2023. "Molecular Physiological Evidence for the Role of Na+-Cl− Co-Transporter in Branchial Na+ Uptake in Freshwater Teleosts" International Journal of Molecular Sciences 24, no. 7: 6597. https://doi.org/10.3390/ijms24076597