Lung Epithelial Cells from Obese Patients Have Impaired Control of SARS-CoV-2 Infection
Abstract
:1. Introduction
2. Results
2.1. Increased Viral Detection and Replicates in Bronchial Epithelial Cells from Obese Subjects
2.2. Enrichment of Lipid Metabolism-Related Pathways in Infected Bronchial Epithelial Cells from Obese Subjects
2.3. LPIN2 as a Unique DEG in Infected Bronchial Epithelial Cells from Obese Subjects
2.4. Bronchial Epithelial Cells from Obese Subjects Mount a Stronger Cytokine Response upon SARS-CoV-2 Infection at Early Stages of Infection
2.5. Upregulated ACE2 Expression in Bronchial Epithelial Cells from Obese Subjects
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. SARS-CoV-2 Infection of NHBE Cells
4.3. RNA Extraction
4.4. RNA Sequencing
4.5. Identification of Differentially Expressed Genes (DEGs)
4.6. Inflammatory Marker Quantification Using qRT-PCR
4.7. Statistics
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Non-Obese | Obese | |
---|---|---|
N | 4 | 4 |
Age, y | 42.3 ± 7.5 | 32 ± 6.6 |
BMI, kg/m2 | 23.6 ± 5.3 | 37.1 ± 2.8 |
Sex (Male/female) | 2/2 | 3/1 |
Primer Name | Oligo Sequence (5′ to 3′) |
---|---|
SARS-CoV2 N-F | AAGCTGGACTTCCCTATGGTG |
SARS-CoV2 N-R | CGATTGCAGCATTGTTAGCAGG |
SARS2-UpE-F | ATTGTTGATGAGCCTGAAG |
SARS2-UpE-R | TTCGTACTCATCAGCTTG |
ACE2-F | TACTGTGACCCCGCATCTCT |
ACE2-R | TCCAACAGTTTCTGTCCAGC |
IL-1β-F | TACATCAGCACCTCTCAAGCA |
IL-1β-R | CCACATTCAGCACAGGACTCT |
GAPDH-F | GAAGGTGAAGGTCGGAGT |
GAPDH-R | GAAGATGGTGATGGGATTTC |
IL-17A-F | GAGGACAAGAACTTCCCCCG |
IL-17A-R | CATTGCCGTGGAGATTCCAAG |
IFN-β-F | CTTGGATTCCTACAAAGAAGCAGC |
IFN-β-R | TCCTCCTTCTGGAACTGCTGCA |
IL-8-F | TCTGCAGCTCTGTGTGAAGGTG |
IL-8-R | AATTTCTGTGTTGGCGCAGTG |
IL-6-F | ACCTTCCAAAGATGGCTGAAA |
IL-6-R | GCTCTGGCTTGTTCCCTCACTAC |
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Gaudet, M.; Kaufmann, E.; Jalaleddine, N.; Mogas, A.; Hachim, M.; Senok, A.; Divangahi, M.; Hamid, Q.; Al Heialy, S. Lung Epithelial Cells from Obese Patients Have Impaired Control of SARS-CoV-2 Infection. Int. J. Mol. Sci. 2023, 24, 6729. https://doi.org/10.3390/ijms24076729
Gaudet M, Kaufmann E, Jalaleddine N, Mogas A, Hachim M, Senok A, Divangahi M, Hamid Q, Al Heialy S. Lung Epithelial Cells from Obese Patients Have Impaired Control of SARS-CoV-2 Infection. International Journal of Molecular Sciences. 2023; 24(7):6729. https://doi.org/10.3390/ijms24076729
Chicago/Turabian StyleGaudet, Mellissa, Eva Kaufmann, Nour Jalaleddine, Andrea Mogas, Mahmood Hachim, Abiola Senok, Maziar Divangahi, Qutayba Hamid, and Saba Al Heialy. 2023. "Lung Epithelial Cells from Obese Patients Have Impaired Control of SARS-CoV-2 Infection" International Journal of Molecular Sciences 24, no. 7: 6729. https://doi.org/10.3390/ijms24076729