Diagnostic Aqueous Humor Proteome Predicts Metastatic Potential in Uveal Melanoma
Abstract
:1. Introduction
2. Results
2.1. Patient Clinical Characteristics and Demographics
2.2. Target Protein Data Distribution
2.3. Differentially Expression Proteins between GEP2 and GEP1 AH Samples
2.4. Biological Significance of the DEPs
3. Discussion
4. Materials and Methods
4.1. Patient Clinical Characteristics and Demographics
4.2. Specimen Collection and Storage
4.3. Quantification of Protein Expression by Proximity Extension Assay (PEA)
4.4. Gene Ontology Analysis and Annotations
4.5. Statistical Analysis
4.6. Analysis of Somatic Copy Number Alterations (SCNAs)
5. Conclusions
6. Patents
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Characteristic | GEP1, n = 12 | GEP2, n = 5 | p |
---|---|---|---|
Sex (Fisher), n (%) | 0.600 | ||
Females | 7 (58.3) | 4 (80) | |
Males | 5 (41.7) | 1 (20) | |
Eye (Fisher), n (%) | 0.620 | ||
OD | 7 (58.3) | 2 (40.0) | |
OS | 5 (41.7) | 3 (60.0) | |
Age at diagnosis, mean (± SD) (MWU) | 53.3 (15.5) | 62.8 (12.7) | 0.183 |
Eye Color (Fisher), n (%) | 0.620 | ||
Light (blue, gray, green, hazel) | 7 (58.3) | 2 (40.0) | |
Dark (brown) | 5 (41.7) | 3 (60.0) | |
Anterior Involvement (Iris and/or ciliary) (Fisher), n (%) | 0.338 | ||
Yes | 6 (50.0) | 1 (20.0) | |
No | 6 (50.0) | 4 (80.0) | |
AJCC Stage (Linear-by-Linear association), n (%) | 0.012 | ||
I | 6 (50.0) | 0 (0) | |
IIA | 4 (33.3) | 2 (40.0) | |
IIB | 2 (16.7) | 1 (20.0) | |
IIIA, IIIB, IIIC | 0 (0) | 2 (40.0) | |
IV | 0 (0) | 0 (0) | |
PRAME Status, known in 17 cases (Fisher), n (%) | 0.515 | ||
Negative | 11 (91.7) | 4 (80.0) | |
Positive | 1 (8.3) | 1 (20.0) | |
GEP Class, known in 17 cases (Fisher), n (%) | <0.001 | ||
1 | 12 (100.0) | 0 (0) | |
2 | 0 (0) | 5 (80.0) | |
Tumor Stage (Linear-by-Linear association), n (%) | 0.007 | ||
T1 | 9 (75.0) | 0 (0) | |
T2 | 2 (16.7) | 3 (60.0) | |
T3 | 1 (8.3) | 1 (20.0) | |
T4 | 0 (0) | 1 (20.0) | |
BAP1 mutation status, known in 16 cases (Fisher), n (%) | 0.018 | ||
Mutation | 0 (0) | 3 (60.0) | |
Wild type | 11 (100.0) | 2 (40.0) |
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Peng, C.-C.; Sirivolu, S.; Pike, S.; Kim, M.E.; Reiser, B.; Li, H.-T.; Liang, G.; Xu, L.; Berry, J.L. Diagnostic Aqueous Humor Proteome Predicts Metastatic Potential in Uveal Melanoma. Int. J. Mol. Sci. 2023, 24, 6825. https://doi.org/10.3390/ijms24076825
Peng C-C, Sirivolu S, Pike S, Kim ME, Reiser B, Li H-T, Liang G, Xu L, Berry JL. Diagnostic Aqueous Humor Proteome Predicts Metastatic Potential in Uveal Melanoma. International Journal of Molecular Sciences. 2023; 24(7):6825. https://doi.org/10.3390/ijms24076825
Chicago/Turabian StylePeng, Chen-Ching, Shreya Sirivolu, Sarah Pike, Mary E. Kim, Bibiana Reiser, Hong-Tao Li, Gangning Liang, Liya Xu, and Jesse L. Berry. 2023. "Diagnostic Aqueous Humor Proteome Predicts Metastatic Potential in Uveal Melanoma" International Journal of Molecular Sciences 24, no. 7: 6825. https://doi.org/10.3390/ijms24076825