Human Sinoatrial Node Pacemaker Activity: Role of the Slow Component of the Delayed Rectifier K+ Current, IKs
Abstract
:1. Introduction
2. Results
2.1. KCNQ1/KCNE1 Current in Response to Square Voltage Clamp Steps
2.2. KCNQ1/KCNE1 Current in Response to Human SAN-like AP Waveforms
2.3. Data on KCNQ1/KCNE1 Current Incorporated into Human SAN Cell Model
2.4. Dynamics of KCNQ1/KCNE1 Encoded IKs in Human SAN Cell Model
2.5. Bradycardic Effect of F279I Gain-of-Function Mutation in KCNQ1
3. Discussion
3.1. Overview
3.2. Computer Simulations with KCNQ1/KCNE1 Current Based IKs
3.3. Simulating Gain-of-Function Mutations in KCNQ1
3.4. Clinical Observations
3.5. Limitations
4. Materials and Methods
4.1. Cell Preparations
4.2. Data Acquisition
4.3. Square-Step Voltage Clamp Experiments
4.4. Action Potential Clamp Experiments
4.5. Drugs
4.6. Computer Simulations
4.7. Statistics
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Verkerk, A.O.; Wilders, R. Human Sinoatrial Node Pacemaker Activity: Role of the Slow Component of the Delayed Rectifier K+ Current, IKs. Int. J. Mol. Sci. 2023, 24, 7264. https://doi.org/10.3390/ijms24087264
Verkerk AO, Wilders R. Human Sinoatrial Node Pacemaker Activity: Role of the Slow Component of the Delayed Rectifier K+ Current, IKs. International Journal of Molecular Sciences. 2023; 24(8):7264. https://doi.org/10.3390/ijms24087264
Chicago/Turabian StyleVerkerk, Arie O., and Ronald Wilders. 2023. "Human Sinoatrial Node Pacemaker Activity: Role of the Slow Component of the Delayed Rectifier K+ Current, IKs" International Journal of Molecular Sciences 24, no. 8: 7264. https://doi.org/10.3390/ijms24087264