A Review of Novel Strategies for Human Periodontal Ligament Stem Cell Ex Vivo Expansion: Are They an Evidence-Based Promise for Regenerative Periodontal Therapy?
Abstract
:1. hPDLSCs as Therapeutic Tool: Current Status
2. Pharmacological Approaches to Improve hPDLSC Expansion
2.1. Cytokines and Growth Factors
2.2. Chemicals
2.3. Natural Compounds
2.4. Extracellular Matrix (ECM)
3. Human Blood Derivatives and Allogenic Serum Supplements as New Frontiers in In Vitro hPDLSC Expansion
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Compounds Supplemented to Culture Media for Healthy PDLSCs | Stemness | Expansion | Osteoblastic Differentation | Ref. | |
---|---|---|---|---|---|
Cytokins and Growth Factors | FGF2 (10 ng/mL) and A83-01 (5 μM) | Zhang et al., 2019 [37] | |||
VEGF (25 ng/mL) | Lee et al., 2012 [38] | ||||
bFGF (25 ng/mL) + BMP-2 (50 ng/mL) | Kang et al., 2019 [39] | ||||
IGF (100 ng/mL) | Yan et al., 2012 [40] | ||||
EHFM medium | Di Vito et al., 2019 [41] | ||||
Platelet-rich fibrin (PRF) + IGF-1 (100 ng/mL) | Li et al., 2018 [42] | ||||
b-FGF-2 (10 μg/mL) + IGF-1 (10 μg/mL) + EGF (10 μg/mL) + NGF (10 μg/mL) | Li et al., 2020 [43] | ||||
Neuregulin-1 (0, 5, 10, and 20 ng/mL) | Li et al., 2022 [44] | ||||
Erythropoietin (0, 5, 10, 20, 50 U/mL) | Zheng., et al. 2019 [45,46] | ||||
SDF-1 (100 and 400 ng/mL) | Du et al., 2012 [47] | ||||
SDF-1α (200 ng/mL) + PTH (50 ng/mL) | Du et al., 2016 [48] | ||||
Chemicals | Simvastatin (0.01 or 0.1μM) | Zhao and Liu et al., 2013 [49] | |||
Metformin (50 nM) | Sanz et al., 2018 Jia et al., 2020 [50,51] | ||||
Y-27632 (10 and 20 μM) | Wang et al., 2017 [52] | ||||
Methylsulfonylmethane (1, 5, 10, 25 or 50 mM) | Ha et al., 2020 [53] | ||||
Sulfonated chitosan oligosaccharide (SCOS) + bFGF (20 ng/mL): molar ratios: 1:1; 1:5; 1:10) | Li et al., 2020 [54] | ||||
Potassium dihydrogen phosphate (1.8 mmol/L) | Xu et al., 2019 [55] | ||||
COMP-Ang1 (300 and 600 ng/mL) | Kook et al., 2014 [56] | ||||
Natural Compounds | Melatonin | Tan et al., 2021 [57] | |||
Progranulin (25 ng/mL) | Yu et al., 2021 [58] | ||||
Rutin (1 × 10−6 mol/L) + vitamin C (20 µg/mL) | Zhao et al., 2019 [59] | ||||
Nicotinamide (0 μM, 50 μM, 100 μM, 200 μM and 300 Μm) | Zheng et al., 2020 [60] | ||||
Naringenin (1–10 uM) | Zhang et al., 2021 [61] | ||||
Z. schinifolium (5, 10, 25, 50, 100 μg/mL) | Kim et al., 2015 [62] | ||||
Myricetin (0.01, 0.1, and 1 μM) | Kim et al., 2018 [63] | ||||
Baicalein (1.25–10 μM) | Chen et al., 2017 [64] | ||||
Baicalein (20, 40, or 80 μM) + LPS (200 ng/mL) | Ren et al., 2021 [65] | ||||
Oxytocin (50 nM) | Ge et al., 2019 [66] | ||||
ECM | UECM (human urine-derived stem cells) | Xiong et al., 2019 [67] | |||
Human Serums | Human platelet lysate (PL) | Wu et al., 2017 [68] | |||
Autologous platelet-rich plasma (PRP) and platelet-poor plasma (PPP) | Martínez et al., 2019 [69] | ||||
House allogeneic human serums (Allo-HS) | Arpornmaeklong et al., 2018 [70] |
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Di Vito, A.; Bria, J.; Antonelli, A.; Mesuraca, M.; Barni, T.; Giudice, A.; Chiarella, E. A Review of Novel Strategies for Human Periodontal Ligament Stem Cell Ex Vivo Expansion: Are They an Evidence-Based Promise for Regenerative Periodontal Therapy? Int. J. Mol. Sci. 2023, 24, 7798. https://doi.org/10.3390/ijms24097798
Di Vito A, Bria J, Antonelli A, Mesuraca M, Barni T, Giudice A, Chiarella E. A Review of Novel Strategies for Human Periodontal Ligament Stem Cell Ex Vivo Expansion: Are They an Evidence-Based Promise for Regenerative Periodontal Therapy? International Journal of Molecular Sciences. 2023; 24(9):7798. https://doi.org/10.3390/ijms24097798
Chicago/Turabian StyleDi Vito, Anna, Jessica Bria, Alessandro Antonelli, Maria Mesuraca, Tullio Barni, Amerigo Giudice, and Emanuela Chiarella. 2023. "A Review of Novel Strategies for Human Periodontal Ligament Stem Cell Ex Vivo Expansion: Are They an Evidence-Based Promise for Regenerative Periodontal Therapy?" International Journal of Molecular Sciences 24, no. 9: 7798. https://doi.org/10.3390/ijms24097798