MicroRNA-155-5p Targets JADE-1, Promoting Proliferation, Migration, and Invasion in Clear Cell Renal Cell Carcinoma Cells
Abstract
:1. Introduction
2. Results
2.1. Survival Analyses
2.2. Knockdown of miR-155-5p and Jade-1
2.3. miR-155-5p Directly Targets the 3′UTR of Jade-1
2.4. miR-155-5p and Jade-1 Affect Phenotypes Associated with Metastasis in ccRCC Cell Lines
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Identification of miR-155-5p Protein Targets
4.3. Knockdown of miR-155 by CRISPR/Cas9
4.4. Lentiviral Transduction of ccRCC Cells with shRNA and Anti-miR
4.5. Real-Time PCR of miR-155-5p Expression
4.6. Cell Proliferation Assay
4.7. Cell Migration and Invasion Assays
4.8. Xenograft Model
4.9. Luciferase Assay
4.10. Western Blot Analysis
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kalantzakos, T.; Hooper, K.; Das, S.; Sullivan, T.; Canes, D.; Moinzadeh, A.; Rieger-Christ, K. MicroRNA-155-5p Targets JADE-1, Promoting Proliferation, Migration, and Invasion in Clear Cell Renal Cell Carcinoma Cells. Int. J. Mol. Sci. 2023, 24, 7825. https://doi.org/10.3390/ijms24097825
Kalantzakos T, Hooper K, Das S, Sullivan T, Canes D, Moinzadeh A, Rieger-Christ K. MicroRNA-155-5p Targets JADE-1, Promoting Proliferation, Migration, and Invasion in Clear Cell Renal Cell Carcinoma Cells. International Journal of Molecular Sciences. 2023; 24(9):7825. https://doi.org/10.3390/ijms24097825
Chicago/Turabian StyleKalantzakos, Thomas, Kailey Hooper, Sanjna Das, Travis Sullivan, David Canes, Alireza Moinzadeh, and Kimberly Rieger-Christ. 2023. "MicroRNA-155-5p Targets JADE-1, Promoting Proliferation, Migration, and Invasion in Clear Cell Renal Cell Carcinoma Cells" International Journal of Molecular Sciences 24, no. 9: 7825. https://doi.org/10.3390/ijms24097825