Next Article in Journal
Protection against Ischemic Heart Disease: A Joint Role for eNOS and the KATP Channel
Next Article in Special Issue
Wheat ABA Receptor TaPYL5 Constitutes a Signaling Module with Its Downstream Partners TaPP2C53/TaSnRK2.1/TaABI1 to Modulate Plant Drought Response
Previous Article in Journal
The Role of NAD+, SIRTs Interactions in Stimulating and Counteracting Carcinogenesis
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 24
Issue 9
10.3390/ijms24097926
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

The miR166d/TaCPK7-D Signaling Module Is a Critical Mediator of Wheat (Triticum aestivum L.) Tolerance to K+ Deficiency

by
Xiaotong Lei
†,
Miaomiao Chen
†,
Ke Xu
,
Ruoxi Sun
,
Sihang Zhao
,
Ningjing Wu
,
Shuhua Zhang
,
Xueju Yang
,
Kai Xiao
* and
Yong Zhao
*
State Key Laboratory of North China Crop Improvement and Regulation, North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Key Laboratory for Crop Germplasm Resources of Hebei, Hebei Agricultural University, Baoding 071000, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2023, 24(9), 7926; https://doi.org/10.3390/ijms24097926
Submission received: 28 March 2023 / Revised: 21 April 2023 / Accepted: 25 April 2023 / Published: 27 April 2023
(This article belongs to the Special Issue Molecular Advances in Resistance and Adaptability of Wheat Crops)
Browse Figures
Versions Notes

Abstract

It is well established that potassium (K+) is an essential nutrient for wheat (Triticum aestivum L.) growth and development. Several microRNAs (miRNAs), including miR166, are reportedly vital roles related to plant growth and stress responses. In this study, a K+ starvation-responsive miRNA (miR166d) was identified, which showed increased expression in the roots of wheat seedlings exposed to low-K+ stress. The overexpression of miR166d considerably increased the tolerance of transgenic Arabidopsis plants to K+ deprivation treatment. Furthermore, disrupting miR166d expression via virus-induced gene silencing (VIGS) adversely affected wheat adaptation to low-K+ stress. Additionally, miR166d directly targeted the calcium-dependent protein kinase 7-D gene (TaCPK7-D) in wheat. The TaCPK7-D gene expression was decreased in wheat seedling roots following K+ starvation treatment. Silencing TaCPK7-D in wheat increased K+ uptake under K+ starvation. Moreover, we observed that the miR166d/TaCPK7-D module could affect wheat tolerance to K+ starvation stress by regulating TaAKT1 and TaHAK1 expression. Taken together, our results indicate that miR166d is vital for K+ uptake and K+ starvation tolerance of wheat via regulation of TaCPK7-D.
Keywords: wheat (Triticum aestivum L.); microRNA166d; calcium-dependent protein kinases 7-D (TaCPK7-D); potassium starvation; potassium acquisition wheat (Triticum aestivum L.); microRNA166d; calcium-dependent protein kinases 7-D (TaCPK7-D); potassium starvation; potassium acquisition

Share and Cite

MDPI and ACS Style

Lei, X.; Chen, M.; Xu, K.; Sun, R.; Zhao, S.; Wu, N.; Zhang, S.; Yang, X.; Xiao, K.; Zhao, Y. The miR166d/TaCPK7-D Signaling Module Is a Critical Mediator of Wheat (Triticum aestivum L.) Tolerance to K+ Deficiency. Int. J. Mol. Sci. 2023, 24, 7926. https://doi.org/10.3390/ijms24097926

AMA Style

Lei X, Chen M, Xu K, Sun R, Zhao S, Wu N, Zhang S, Yang X, Xiao K, Zhao Y. The miR166d/TaCPK7-D Signaling Module Is a Critical Mediator of Wheat (Triticum aestivum L.) Tolerance to K+ Deficiency. International Journal of Molecular Sciences. 2023; 24(9):7926. https://doi.org/10.3390/ijms24097926

Chicago/Turabian Style

Lei, Xiaotong, Miaomiao Chen, Ke Xu, Ruoxi Sun, Sihang Zhao, Ningjing Wu, Shuhua Zhang, Xueju Yang, Kai Xiao, and Yong Zhao. 2023. "The miR166d/TaCPK7-D Signaling Module Is a Critical Mediator of Wheat (Triticum aestivum L.) Tolerance to K+ Deficiency" International Journal of Molecular Sciences 24, no. 9: 7926. https://doi.org/10.3390/ijms24097926

APA Style

Lei, X., Chen, M., Xu, K., Sun, R., Zhao, S., Wu, N., Zhang, S., Yang, X., Xiao, K., & Zhao, Y. (2023). The miR166d/TaCPK7-D Signaling Module Is a Critical Mediator of Wheat (Triticum aestivum L.) Tolerance to K+ Deficiency. International Journal of Molecular Sciences, 24(9), 7926. https://doi.org/10.3390/ijms24097926

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop