Effects of Probiotic Saccharomyces boulardii Supernatant on Viability, Nano-Mechanical Properties of Cytoplasmic Membrane and Pro-Inflammatory Gene Expression in Human Gastric Cancer AGS Cells
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Saccharomyces Cerevisiae var. Boulardii Supernatant (SBS) Preparation
4.2. Cell Culture
4.3. Cell Viability
4.4. Atomic Force Microscopy Analysis
4.5. Expression of Survivin and Pro-Inflammatory Genes
4.6. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment | Mean Young’s Modulus Value (kpa) ± SD * | Mean Adhesion Force (pN) ± SD | Mean Z Pulling (µm) ± SD |
---|---|---|---|
Control | 0.95 ± 0.025 a | 148 ± 4.02 a | 0.61 ± 0.012 a |
After 24 h | 1.57 ± 0.180 b | 128 ± 5.20 b | 1.23 ± 0.120 b |
After 48 h | 1.62 ± 0.220 b | 112 ± 4.20 b | 1.58 ± 0.025 b |
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Pakbin, B.; Allahyari, S.; Dibazar, S.P.; Zolghadr, L.; Chermahini, N.K.; Brück, W.M.; Brück, T.B.; Mahmoudi, R. Effects of Probiotic Saccharomyces boulardii Supernatant on Viability, Nano-Mechanical Properties of Cytoplasmic Membrane and Pro-Inflammatory Gene Expression in Human Gastric Cancer AGS Cells. Int. J. Mol. Sci. 2023, 24, 7945. https://doi.org/10.3390/ijms24097945
Pakbin B, Allahyari S, Dibazar SP, Zolghadr L, Chermahini NK, Brück WM, Brück TB, Mahmoudi R. Effects of Probiotic Saccharomyces boulardii Supernatant on Viability, Nano-Mechanical Properties of Cytoplasmic Membrane and Pro-Inflammatory Gene Expression in Human Gastric Cancer AGS Cells. International Journal of Molecular Sciences. 2023; 24(9):7945. https://doi.org/10.3390/ijms24097945
Chicago/Turabian StylePakbin, Babak, Samaneh Allahyari, Shaghayegh Pishkhan Dibazar, Leila Zolghadr, Neda Karami Chermahini, Wolfram Manuel Brück, Thomas B. Brück, and Razzagh Mahmoudi. 2023. "Effects of Probiotic Saccharomyces boulardii Supernatant on Viability, Nano-Mechanical Properties of Cytoplasmic Membrane and Pro-Inflammatory Gene Expression in Human Gastric Cancer AGS Cells" International Journal of Molecular Sciences 24, no. 9: 7945. https://doi.org/10.3390/ijms24097945