Bna.EPF2 Enhances Drought Tolerance by Regulating Stomatal Development and Stomatal Size in Brassica napus
Abstract
:1. Introduction
2. Results
2.1. Isolation and Characterization of Bna.EPF2 in Brassica napus
2.2. Expression Pattern of Bna.EPF2
2.3. The Ectopic Expression of Bna.EPF2 Rescued the Stomatal Development Defects in Arabidopsis epf2 Mutant
2.4. Overexpression of Bna.EPF2 in Brassica Plants Caused a Lower Stomatal Density and Smaller Stomatal Complex Size
2.5. Overexpression of Bna.EPF2 in B. napus-Conferred Enhanced Drought Tolerance
2.6. Overexpression of Bna.EPF2 in B. napus-Improved WUE by Reducing Transpiration
2.7. Overexpression of Bna.EPF2 Did Not Influence Major Agronomic Traits
2.8. Bna.EPF2 Affects Stomatal Density via Different Stomatal Development Pathways in B. napus
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Sequence Retrieval and Phylogenetic Tree Construction
4.3. Generation of pBna.EPF2::GUS Transgenic Lines and GUS Staining
4.4. Generation of Transgenic Arabidopsis and Brassica napus Plants
4.5. Thermal Imaging Analysis
4.6. Transpiration and WUE Measurements
4.7. Stomatal Density and Stomatal Complex Size Measurement
4.8. Drought Stress Assays
4.9. Biomass and Yield Trait Measurements
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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WT (n = 6) | Bna.EPF-17 (n = 6) | BnaEPF-19 (n = 6) | BnaEPF-29 (n = 6) | |
---|---|---|---|---|
Plant height (cm) | 140.67 ± 8.08 a | 142.17 ± 9.87 a | 132.67 ± 8.24 a | 147.75 ± 9.01 a |
(p = 0.5737) | (p = 0.1138) | (p = 0.1919) | ||
Length of main inflorescence (cm) | 42.33 ± 6.53 a | 39.92 ± 5.50 a | 36.67 ± 6.62 a | 41.33 ± 6.28 a |
(p = 0.5039) | (p = 0.1665) | (p = 0.7924) | ||
Number of siliques on main inflorescence | 39.50 ± 4.76 a | 37.83 ± 4.88 a | 37.17 ± 2.48 a | 38.67 ± 5.13 a |
(p = 0.5626) | (p = 0.3124) | (p = 0.7765) | ||
Number of first branch | 9.00 ± 1.79 a | 8.83 ± 1.17 a | 8.67 ± 1.75 a | 8.33 ± 2.25 a |
(p = 0.8523) | (p = 0.7510) | (p = 0.5826) | ||
Length per silique (cm) | 5.16 ± 0.33 a | 5.38 ± 0.28 bc | 5.31 ± 0.25 b | 5.47 ± 0.31 c |
(p = 0.0001) | (p = 0.0049) | (p = 0.00008) | ||
Number of seeds per silique | 24.45 ± 2.79 a | 25.42 ± 2.47 b | 24.73 ± 2.48 ab | 26.15 ± 3.00 b |
(p = 0.0468) | (p = 0.5573) | (p = 0.0017) | ||
1000-seed weight (g) | 3.49 ± 0.34 a | 3.28 ± 0.44 ab | 3.27 ± 0.32 ab | 3.66 ± 0.45 b |
(p = 0.3952) | (p = 0.2797) | (p = 0.0427) | ||
Seed yield per plant (g) | 20.92 ± 1.16 a | 20.52 ± 1.60 a | 20.36 ± 1.77 a | 20.60 ± 1.48 a |
(p = 0.3431) | (p = 0.1748) | (p = 0.1138) |
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Jiao, P.; Liang, Y.; Chen, S.; Yuan, Y.; Chen, Y.; Hu, H. Bna.EPF2 Enhances Drought Tolerance by Regulating Stomatal Development and Stomatal Size in Brassica napus. Int. J. Mol. Sci. 2023, 24, 8007. https://doi.org/10.3390/ijms24098007
Jiao P, Liang Y, Chen S, Yuan Y, Chen Y, Hu H. Bna.EPF2 Enhances Drought Tolerance by Regulating Stomatal Development and Stomatal Size in Brassica napus. International Journal of Molecular Sciences. 2023; 24(9):8007. https://doi.org/10.3390/ijms24098007
Chicago/Turabian StyleJiao, Peipei, Yuanlin Liang, Shaoping Chen, Yang Yuan, Yongqiang Chen, and Honghong Hu. 2023. "Bna.EPF2 Enhances Drought Tolerance by Regulating Stomatal Development and Stomatal Size in Brassica napus" International Journal of Molecular Sciences 24, no. 9: 8007. https://doi.org/10.3390/ijms24098007