Silencing a Chitinase Gene, PstChia1, Reduces Virulence of Puccinia striiformis f. sp. tritici
Abstract
:1. Introduction
2. Results
2.1. Identification of Chitinase Genes in 10 Fungal Genomes
2.2. Expression Analysis of PstChias during Wheat-Pst Interaction
2.3. PstChia1 Has Chitinase Activity
2.4. Silencing of PstChia1 Reduces the Pathogenicity of Pst
2.5. Knockdown of PstChia1 Inhibits the Development of Pst
2.6. Defense-Related Genes Were Influenced by Knockdown of PstChia1
3. Discussion
4. Materials and Methods
4.1. Identification and Phylogenetic Analysis of PstChia Genes
4.2. Analysis of Gene Expression
4.3. Analysis of Gene Transcriptional Levels by Quantitative Real-Time PCR
4.4. Enzyme Activity Assay of Chitinase PstChia1
4.5. BSMV Mediate Host-Induced Gene Silencing
4.6. Histological Observations of Fungal Growth and H2O2 Accumulation
4.7. Analysis of RT-PCR Results for Pathogenesis-Related Protein Genes
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Guo, J.; Mou, Y.; Li, Y.; Yang, Q.; Wang, X.; Lin, H.; Kang, Z.; Guo, J. Silencing a Chitinase Gene, PstChia1, Reduces Virulence of Puccinia striiformis f. sp. tritici. Int. J. Mol. Sci. 2023, 24, 8215. https://doi.org/10.3390/ijms24098215
Guo J, Mou Y, Li Y, Yang Q, Wang X, Lin H, Kang Z, Guo J. Silencing a Chitinase Gene, PstChia1, Reduces Virulence of Puccinia striiformis f. sp. tritici. International Journal of Molecular Sciences. 2023; 24(9):8215. https://doi.org/10.3390/ijms24098215
Chicago/Turabian StyleGuo, Jia, Ying Mou, Yuanxing Li, Qing Yang, Xue Wang, Haocheng Lin, Zhensheng Kang, and Jun Guo. 2023. "Silencing a Chitinase Gene, PstChia1, Reduces Virulence of Puccinia striiformis f. sp. tritici" International Journal of Molecular Sciences 24, no. 9: 8215. https://doi.org/10.3390/ijms24098215