SMYD3 Controls Ciliogenesis by Regulating Distinct Centrosomal Proteins and Intraflagellar Transport Trafficking
Abstract
:1. Introduction
2. Results
2.1. SMYD3 Is Localized at the Basal Body and Regulates Cilia Biogenesis
2.2. SMYD3 Is an Appendage Protein
2.3. SMYD3 Modulates Microtubule Stability
2.4. SMYD3 Regulates Centriolar Appendage Assembly
2.5. SMYD3 Modulates TTBK2 and CP110 Basal Body Localization
2.6. SMYD3 Depletion Affects Ciliary Trafficking of IFT Proteins
2.7. SMYD3 Regulates the Transcription of Cilia-Related Genes
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Plasmids
4.3. Generating Stable SMYD3 Knockdown IMCD3 Cell Line
4.4. Drug Treatment
4.5. Antibodies and Reagents
4.6. Immunofluorescence Staining
4.7. Western Blot Analysis and Immunoprecipitation
4.8. Microscopy and Imaging
4.9. Transmission Electron Microscopy (TEM)
4.10. Real-Time Quantitative Reverse Transcription PCR (qRT-PCR)
4.11. Chromatin Immunoprecipitation (ChIP) Assay
4.12. RNA Interference
4.13. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Agborbesong, E.; Zhou, J.X.; Zhang, H.; Li, L.X.; Harris, P.C.; Calvet, J.P.; Li, X. SMYD3 Controls Ciliogenesis by Regulating Distinct Centrosomal Proteins and Intraflagellar Transport Trafficking. Int. J. Mol. Sci. 2024, 25, 6040. https://doi.org/10.3390/ijms25116040
Agborbesong E, Zhou JX, Zhang H, Li LX, Harris PC, Calvet JP, Li X. SMYD3 Controls Ciliogenesis by Regulating Distinct Centrosomal Proteins and Intraflagellar Transport Trafficking. International Journal of Molecular Sciences. 2024; 25(11):6040. https://doi.org/10.3390/ijms25116040
Chicago/Turabian StyleAgborbesong, Ewud, Julie Xia Zhou, Hongbing Zhang, Linda Xiaoyan Li, Peter C. Harris, James P. Calvet, and Xiaogang Li. 2024. "SMYD3 Controls Ciliogenesis by Regulating Distinct Centrosomal Proteins and Intraflagellar Transport Trafficking" International Journal of Molecular Sciences 25, no. 11: 6040. https://doi.org/10.3390/ijms25116040