Substance P Promotes Leukocyte Infiltration in the Liver and Lungs of Mice with Sepsis: A Key Role for Adhesion Molecules on Vascular Endothelial Cells
Abstract
:1. Introduction
2. Results
2.1. Inhibiting the Biosynthesis of SP or Blocking NK1R Mitigated the Elevation in the Activity of MPO in the Liver of Mice with Sepsis Induced by CLP Surgery
2.2. Inhibiting the Biosynthesis of SP or Blocking NK1R Attenuated the Increase in the Activity of MPO in the Lungs of Mice with Sepsis Induced by CLP Surgery
2.3. Inhibiting the Biosynthesis of SP or Blocking NK1R Attenuated the Increased Concentrations of ICAM1 and VCAM1 in the Liver of Mice with Sepsis Induced by CLP Surgery
2.4. Inhibiting the Biosynthesis of SP or Blocking NK1R Attenuated the Increases in the Concentrations of ICAM1 and VCAM1 in the Lungs of Mice with Sepsis Induced by CLP Surgery
2.5. Inhibiting the Biosynthesis of SP or Blocking NK1R Attenuated the Upregulation of the Expression of ICAM1 and VCAM1 on the Liver Sinusoidal Endothelial Cells of Mice with Sepsis Induced by CLP Surgery
2.6. Inhibiting the Biosynthesis of SP or Blocking NK1R Attenuated the Upregulation of the Expression of ICAM1 and VCAM1 on the Pulmonary Endothelial Cells of Mice with Sepsis Induced by CLP Surgery
3. Discussion
4. Materials and Methods
4.1. Group Setting
4.2. Sepsis Establishment, Manipulations, and Sample Collection from Mice
4.3. Measurement of the Activity of MPO in Tissues
4.4. Measurement of the Concentrations of ICAM1 and VCAM1 in Tissues
4.5. Measurement of the Expression of ICAM1 and VCAM1 on Vascular Endothelial Cells in Tissues by Double Immunofluorescence Staining
4.6. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Antibody | Dilution | Source, Catalogue No. |
---|---|---|
ICAM1 | 1:1000 | R&D Systems, Minneapolis, MN, USA |
VCAM1 | 1:200 | R&D Systems, Minneapolis, MN, USA |
LYVE1 | 1:200 | Abcam, Cambridge, UK |
CD31 | 1:100 | Abcam, Cambridge, UK |
FITC-conjugated secondary antibody | 1:500 | Abcam, Cambridge, UK |
Texas red-conjugated secondary antibody | 1:500 | Abcam, Cambridge, UK |
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Zhu, Z.; Chambers, S.; Bhatia, M. Substance P Promotes Leukocyte Infiltration in the Liver and Lungs of Mice with Sepsis: A Key Role for Adhesion Molecules on Vascular Endothelial Cells. Int. J. Mol. Sci. 2024, 25, 6500. https://doi.org/10.3390/ijms25126500
Zhu Z, Chambers S, Bhatia M. Substance P Promotes Leukocyte Infiltration in the Liver and Lungs of Mice with Sepsis: A Key Role for Adhesion Molecules on Vascular Endothelial Cells. International Journal of Molecular Sciences. 2024; 25(12):6500. https://doi.org/10.3390/ijms25126500
Chicago/Turabian StyleZhu, Zhixing, Stephen Chambers, and Madhav Bhatia. 2024. "Substance P Promotes Leukocyte Infiltration in the Liver and Lungs of Mice with Sepsis: A Key Role for Adhesion Molecules on Vascular Endothelial Cells" International Journal of Molecular Sciences 25, no. 12: 6500. https://doi.org/10.3390/ijms25126500