Development of Bioactive Hybrid Poly(lactic acid)/Poly(methyl methacrylate) (PLA/PMMA) Electrospun Fibers Functionalized with Bioglass Nanoparticles for Bone Tissue Engineering Applications
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of Bioglass Nanoparticle (n-BG)
2.2. Morphology and Size of Hybrid Electrospun Fibers
2.3. Wettability of Hybrid Electrospun Fibers
2.4. Infrared Spectroscopy Analysis of the Hybrid Electrospun Fibers
2.5. Mechanical Characterization of Hybrid Fibers
2.6. In Vitro Bioactivity
2.7. In Vitro Hydrolytic Degradation
2.8. In Vitro Cell Viability
2.9. In Vivo Biocompatibility
3. Materials and Methods
3.1. Materials
3.2. Preparation of Hybrid PLA/PMMA and PLA/PMMA/n-BG Fibers via Electrospinning Technique
3.3. Characterization of n-BG Nanoparticles, Hybrid PLA/PMMA, and PLA/PMMA/n-BG Fibers
3.3.1. Nanoparticle Characterization
3.3.2. Morphology and Size of Hybrid Electrospun Fibers
3.3.3. Wettability of the Hybrid Electrospun Fibers
3.3.4. Infrared Spectroscopy Analysis of the Hybrid Electrospun Fibers
3.3.5. Mechanical Characterization of Hybrid Fibers
3.3.6. In Vitro Bioactivity in Simulated Body Fluid (SBF)
3.3.7. In Vitro Hydrolytic Degradation in Phosphate-Buffered Saline Solution (PBS)
3.3.8. In Vitro Cell Viability
3.3.9. In Vivo Biocompatibility
3.3.10. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample | Ca (% wt./wt.) | P (% wt./wt.) | Ca/P Relation |
---|---|---|---|
PP01 | 0 | 0 | 0 |
PP02 | 0 | 0 | 0 |
PP03 | 0 | 0 | 0 |
PPBG01 | 32.5 | 13.3 | 1.90 |
PPBG02 | 35.5 | 13.2 | 2.06 |
PPBG03 | 24.3 | 10.0 | 1.87 |
Sample | Code | Amount PLA [g] | Amount PMMA [g] | Amount n-BG [g] |
---|---|---|---|---|
Neat PLA | PP-01 | 1.250 | 0 | 0 |
PLA/PMMA (75/25) | PP-02 | 0.9375 | 0.3125 | 0 |
PLA/PMMA (50/50) | PP-03 | 0.6250 | 0.6250 | 0 |
PLA/PMMA (25/75) | PP-04 | 0.3125 | 0.9375 | 0 |
Neat PMMA | PP-05 | 0 | 1.250 | 0 |
PLA + 10 wt.% n-BG | PPBG-01 | 1.125 | 0 | 0.1250 |
PLA/PMMA (75/25) + 10 wt.% n-BG | PPBG-02 | 0.8437 | 0.2813 | 0.1250 |
PLA/PMMA (50/50) + 10 wt.% n-BG | PPBG-03 | 0.5625 | 0.5625 | 0.1250 |
PLA/PMMA (25/75) + 10 wt.% n-BG | PPBG-04 | 0.2813 | 0.8437 | 0.1250 |
PMMA + 10 wt.% n-BG | PPBG-05 | 0 | 1.125 | 0.1250 |
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Álvarez-Carrasco, F.; Varela, P.; Sarabia-Vallejos, M.A.; García-Herrera, C.; Saavedra, M.; Zapata, P.A.; Zárate-Triviño, D.; Martínez, J.J.; Canales, D.A. Development of Bioactive Hybrid Poly(lactic acid)/Poly(methyl methacrylate) (PLA/PMMA) Electrospun Fibers Functionalized with Bioglass Nanoparticles for Bone Tissue Engineering Applications. Int. J. Mol. Sci. 2024, 25, 6843. https://doi.org/10.3390/ijms25136843
Álvarez-Carrasco F, Varela P, Sarabia-Vallejos MA, García-Herrera C, Saavedra M, Zapata PA, Zárate-Triviño D, Martínez JJ, Canales DA. Development of Bioactive Hybrid Poly(lactic acid)/Poly(methyl methacrylate) (PLA/PMMA) Electrospun Fibers Functionalized with Bioglass Nanoparticles for Bone Tissue Engineering Applications. International Journal of Molecular Sciences. 2024; 25(13):6843. https://doi.org/10.3390/ijms25136843
Chicago/Turabian StyleÁlvarez-Carrasco, Fabián, Pablo Varela, Mauricio A. Sarabia-Vallejos, Claudio García-Herrera, Marcela Saavedra, Paula A. Zapata, Diana Zárate-Triviño, Juan José Martínez, and Daniel A. Canales. 2024. "Development of Bioactive Hybrid Poly(lactic acid)/Poly(methyl methacrylate) (PLA/PMMA) Electrospun Fibers Functionalized with Bioglass Nanoparticles for Bone Tissue Engineering Applications" International Journal of Molecular Sciences 25, no. 13: 6843. https://doi.org/10.3390/ijms25136843