GCPII Inhibition Promotes Remyelination after Peripheral Nerve Injury in Aged Mice
Abstract
:1. Introduction
2. Results
2.1. GCPII Activity and Expression Are Elevated in Peripheral Nerves after Injury
2.2. GCPII Inhibition with 2-PMPA Promotes Myelination In Vitro
2.3. GCPII Inhibition Accelerates Remyelination after PNI in 3-Month-Old Mice
2.4. GCPII Inhibition Enhances Remyelination after PNI in 20-Month-Old Mice
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Sciatic Nerve Injury
4.3. GCPII Enzyme Activity Assay
4.4. Immunofluorescent Staining
4.5. Dorsal Root Ganglia Explants
4.6. Morphological Analyses of Nerves
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Target | Primary Antibody | Secondary Antibody |
---|---|---|
GCPII | GCP-04 (Goat anti-Mouse, 1:250) (Novus, NBP1-45057, Centennial, CO, USA) | 647 nm, anti-mouse IgG1 (1:1000) (Invitrogen, A66784, Waltham, MA, USA) |
Repair Schwann cells | GFAP (Goat anti-Chicken, 1:250) (Abcam, Ab4674, Waltham, MA, USA) | 488 nm, anti-chicken IgG (H + L) (1:1000) (Invitrogen, A11039, Waltham, MA, USA) |
Myelin Schwann cells | S100 (Goat anti-Rabbit, 1:25) (DAKO, GA50461-2, Santa Clara, CA, USA) | 546 nm, anti-rabbit IgG (H + L) (1:1000) (Invitrogen, A11035, Waltham, MA, USA) |
Macrophages | CD68 (Goat anti-Rat, 1:250) (Invitrogen, PA5-109344, Waltham, MA, USA) | 405 nm, anti-rat IgG (H + L) (1:1000) (Invitrogen, A48261, Waltham, MA, USA) |
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Su, Y.; Huang, M.; Thomas, A.G.; Maragakis, J.; Huizar, K.D.J.; Zheng, Y.; Wu, Y.; Farah, M.H.; Slusher, B.S. GCPII Inhibition Promotes Remyelination after Peripheral Nerve Injury in Aged Mice. Int. J. Mol. Sci. 2024, 25, 6893. https://doi.org/10.3390/ijms25136893
Su Y, Huang M, Thomas AG, Maragakis J, Huizar KDJ, Zheng Y, Wu Y, Farah MH, Slusher BS. GCPII Inhibition Promotes Remyelination after Peripheral Nerve Injury in Aged Mice. International Journal of Molecular Sciences. 2024; 25(13):6893. https://doi.org/10.3390/ijms25136893
Chicago/Turabian StyleSu, Yu, Meixiang Huang, Ajit G. Thomas, John Maragakis, Kaitlyn D. J. Huizar, Yuxin Zheng, Ying Wu, Mohamed H. Farah, and Barbara S. Slusher. 2024. "GCPII Inhibition Promotes Remyelination after Peripheral Nerve Injury in Aged Mice" International Journal of Molecular Sciences 25, no. 13: 6893. https://doi.org/10.3390/ijms25136893