Next Article in Journal
Mannan-Binding Lectin Is Associated with Inflammation and Kidney Damage in a Mouse Model of Type 2 Diabetes
Previous Article in Journal
Implications of Genetic Factors Underlying Mouse Hydronephrosis: Cautionary Considerations on Phenotypic Interpretation in Genetically Engineered Mice
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 25
Issue 13
10.3390/ijms25137200
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Recapitulation of HIV-1 Neutralization Breadth in Plasma by the Combination of Two Broadly Neutralizing Antibodies from Different Lineages in the Same SHIV-Infected Rhesus Macaque

by
Yanxin Gai
1,
Nan Gao
1,
Zhaoyang Mou
1,
Chumeng Yang
1,
Libian Wang
1,
Wanshan Ji
1,
Tiejun Gu
1,
Bin Yu
1,
Chu Wang
1,
Xianghui Yu
1,2,* and
Feng Gao
1,3,4,*
1
National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China
2
Key Laboratory for Molecular Enzymology and Engineering, the Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China
3
Institute of Molecular and Medical Virology, School of Medicine, Jinan University, Guangzhou 510632, China
4
Key Laboratory of Viral Pathogenesis & Infection Prevention and Control, Jinan University, Ministry of Education, Guangzhou 510632, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(13), 7200; https://doi.org/10.3390/ijms25137200
Submission received: 20 May 2024 / Revised: 18 June 2024 / Accepted: 28 June 2024 / Published: 29 June 2024
(This article belongs to the Section Molecular Immunology)
Versions Notes

Abstract

Viral infection generally induces polyclonal neutralizing antibody responses. However, how many lineages of antibody responses can fully represent the neutralization activities in sera has not been well studied. Using the newly designed stable HIV-1 Env trimer as hook, we isolated two distinct broadly neutralizing antibodies (bnAbs) from Chinese rhesus macaques infected with SHIV1157ipd3N4 for 5 years. One lineage of neutralizing antibodies (JT15 and JT16) targeted the V2-apex in the Env trimers, similar to the J038 lineage bnAbs identified in our previous study. The other lineage neutralizing antibody (JT18) targeted the V3 crown region in the Env, which strongly competed with human 447-52D. Each lineage antibody neutralized a different set of viruses. Interestingly, when the two neutralizing antibodies from different lineages isolated from the same macaque were combined, the mixture had a neutralization breath very similar to that from the cognate sera. Our study demonstrated that a minimum of two different neutralizing antibodies can fully recapitulate the serum neutralization breadth. This observation can have important implications in AIDS vaccine design.
Keywords: neutralization; rhesus macaque; vaccine; HIV-1; simian-human immunodeficiency virus; broadly neutralizing antibodies neutralization; rhesus macaque; vaccine; HIV-1; simian-human immunodeficiency virus; broadly neutralizing antibodies

Share and Cite

MDPI and ACS Style

Gai, Y.; Gao, N.; Mou, Z.; Yang, C.; Wang, L.; Ji, W.; Gu, T.; Yu, B.; Wang, C.; Yu, X.; et al. Recapitulation of HIV-1 Neutralization Breadth in Plasma by the Combination of Two Broadly Neutralizing Antibodies from Different Lineages in the Same SHIV-Infected Rhesus Macaque. Int. J. Mol. Sci. 2024, 25, 7200. https://doi.org/10.3390/ijms25137200

AMA Style

Gai Y, Gao N, Mou Z, Yang C, Wang L, Ji W, Gu T, Yu B, Wang C, Yu X, et al. Recapitulation of HIV-1 Neutralization Breadth in Plasma by the Combination of Two Broadly Neutralizing Antibodies from Different Lineages in the Same SHIV-Infected Rhesus Macaque. International Journal of Molecular Sciences. 2024; 25(13):7200. https://doi.org/10.3390/ijms25137200

Chicago/Turabian Style

Gai, Yanxin, Nan Gao, Zhaoyang Mou, Chumeng Yang, Libian Wang, Wanshan Ji, Tiejun Gu, Bin Yu, Chu Wang, Xianghui Yu, and et al. 2024. "Recapitulation of HIV-1 Neutralization Breadth in Plasma by the Combination of Two Broadly Neutralizing Antibodies from Different Lineages in the Same SHIV-Infected Rhesus Macaque" International Journal of Molecular Sciences 25, no. 13: 7200. https://doi.org/10.3390/ijms25137200

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop