Deletion of the Mitochondrial Membrane Protein Fam210b Is Associated with the Development of Systemic Lupus Erythematosus
Abstract
:1. Introduction
2. Results
2.1. Fam210b Knockout Induced Lupus-Like Symptoms in Mice
2.2. Fam210b Is Mainly Expressed in Erythroid Cells
2.3. Fam210b Knockout Inhibited Splenic Erythrocyte Differentiation and Development
2.4. Fam210b−/− Erythroid Cells Stimulate Lymphocyte Activation
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Extraction of Peripheral Blood and Spleens from Mice
4.3. Single-Cell Sequencing of Spleens
4.4. Flow Cytometry
4.5. ELISA Assay
4.6. H&E Staining and Immunofluorescence
4.7. CD71+ Erythroid Cell Sorting
4.8. Co-Culture and Stimulation
4.9. dsDNA Content Detection
4.10. ROS Content Detection
4.11. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
List of Abbreviations
SLE | systemic lupus erythematosus |
mtDNA | mitochondrial DNA |
anti-dsDNA | anti-double-stranded DNA |
LPS | lipopolysaccharide |
ANA | anti-nuclear antibody |
TLRs | toll-like receptors |
IFNs | type I interferons |
NETs | neutrophil extracellular traps |
pDCs | plasmacytoid dendritic cells |
ROS | reactive oxygen species |
GWAS | genome-wide association studies |
ATP | adenosine triphosphate |
IRGM | immune-related guanosine triphosphatase M protein |
ND | NADH dehydrogenase |
Fam210b−/− | Fam210b knockout |
ERK | extracellular signal-regulated kinase |
ELISA | enzyme-linked immunosorbent assay |
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Xu, Y.; Gao, R.; Zhang, M.; Zeng, Q.; Zhu, G.; Qiu, J.; Su, W.; Wang, R. Deletion of the Mitochondrial Membrane Protein Fam210b Is Associated with the Development of Systemic Lupus Erythematosus. Int. J. Mol. Sci. 2024, 25, 7253. https://doi.org/10.3390/ijms25137253
Xu Y, Gao R, Zhang M, Zeng Q, Zhu G, Qiu J, Su W, Wang R. Deletion of the Mitochondrial Membrane Protein Fam210b Is Associated with the Development of Systemic Lupus Erythematosus. International Journal of Molecular Sciences. 2024; 25(13):7253. https://doi.org/10.3390/ijms25137253
Chicago/Turabian StyleXu, Yaqi, Ran Gao, Min Zhang, Qi Zeng, Gaizhi Zhu, Jinming Qiu, Wenting Su, and Renxi Wang. 2024. "Deletion of the Mitochondrial Membrane Protein Fam210b Is Associated with the Development of Systemic Lupus Erythematosus" International Journal of Molecular Sciences 25, no. 13: 7253. https://doi.org/10.3390/ijms25137253