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Open AccessArticle
Bitter Taste Receptor 46 (hTAS2R46) Protects Monocytes/Macrophages from Oxidative Stress
by
Maria Talmon
Maria Talmon 1,*,
Lara Camillo
Lara Camillo 2,
Ilaria Vietti
Ilaria Vietti 2,
Federica Pollastro
Federica Pollastro 1
and
Luigia Grazia Fresu
Luigia Grazia Fresu 2,*
1
Department of Pharmaceutical Sciences, University of Piemonte Orientale, 28100 Novara, Italy
2
Department of Health Sciences, School of Medicine, University of Piemonte Orientale, 28100 Novara, Italy
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(13), 7325; https://doi.org/10.3390/ijms25137325 (registering DOI)
Submission received: 5 June 2024
/
Revised: 28 June 2024
/
Accepted: 1 July 2024
/
Published: 3 July 2024
Abstract
Bitter taste receptors (TAS2Rs) are not only responsible for taste perception in the oral cavity, but are spread throughout the body, generating a widespread chemosensory system. In humans, 25 subtypes have been identified and are differentially expressed in tissues and organs, including in the immune system. In fact, several TAS2R subtypes have been detected in neutrophils, lymphocytes, B and T cells, NK cells, and monocytes/macrophages, in which they regulate various protective functions of the innate immune system. Given its recognized anti-inflammatory and antioxidant activity, and the generally protective role of bitter taste receptors, in this work, we studied TAS2R46’s potential in the protection of human monocyte/macrophage DNA from stress-induced damage. Through both direct and indirect assays and a single-cell gel electrophoresis assay, we demonstrated that absinthin, a specific TAS2R46 agonist, counteracts the release of reactive oxygen species (ROS) and reactive nitrogen species (RNS) and reduces DNA damage in both cell types. Even though the release of ROS from monocytes/macrophages is fundamental for contrast pathogen agents, supraphysiological ROS production impairs their function, finally leading to cell death. Our results highlight TAS2R46 as a novel player involved in the protection of monocytes and macrophages from oxidative stress damage, while simultaneously supporting their antimicrobial activity.
Share and Cite
MDPI and ACS Style
Talmon, M.; Camillo, L.; Vietti, I.; Pollastro, F.; Fresu, L.G.
Bitter Taste Receptor 46 (hTAS2R46) Protects Monocytes/Macrophages from Oxidative Stress. Int. J. Mol. Sci. 2024, 25, 7325.
https://doi.org/10.3390/ijms25137325
AMA Style
Talmon M, Camillo L, Vietti I, Pollastro F, Fresu LG.
Bitter Taste Receptor 46 (hTAS2R46) Protects Monocytes/Macrophages from Oxidative Stress. International Journal of Molecular Sciences. 2024; 25(13):7325.
https://doi.org/10.3390/ijms25137325
Chicago/Turabian Style
Talmon, Maria, Lara Camillo, Ilaria Vietti, Federica Pollastro, and Luigia Grazia Fresu.
2024. "Bitter Taste Receptor 46 (hTAS2R46) Protects Monocytes/Macrophages from Oxidative Stress" International Journal of Molecular Sciences 25, no. 13: 7325.
https://doi.org/10.3390/ijms25137325
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