Next Article in Journal
Chloride Gradient Is Involved in Ammonium Influx in Human Erythrocytes
Previous Article in Journal
The Role of Short Chain Fatty Acids in Inflammation and Body Health
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

The Effects of Sevoflurane and Aβ Interaction on CA1 Dendritic Spine Dynamics and MEGF10-Related Astrocytic Synapse Engulfment

1
Department of Anesthesiology and Intensive Care Medicine, School of Medicine and Health, Klinikum Rechts der Isar, Technical University of Munich, 81675 Munich, Germany
2
Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
3
School of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
4
Graduate School of Systemic Neuroscience, Ludwig Maximilian University of Munich, 82152 Munich, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2024, 25(13), 7393; https://doi.org/10.3390/ijms25137393
Submission received: 3 June 2024 / Revised: 1 July 2024 / Accepted: 2 July 2024 / Published: 5 July 2024

Abstract

General anesthetics may accelerate the neuropathological changes related to Alzheimer’s disease (AD), of which amyloid beta (Aβ)-induced toxicity is one of the main causes. However, the interaction of general anesthetics with different Aβ-isoforms remains unclear. In this study, we investigated the effects of sevoflurane (0.4 and 1.2 maximal alveolar concentration (MAC)) on four Aβ species-induced changes on dendritic spine density (DSD) in hippocampal brain slices of Thy1-eGFP mice and multiple epidermal growth factor-like domains 10 (MEGF10)-related astrocyte-mediated synaptic engulfment in hippocampal brain slices of C57BL/6 mice. We found that both sevoflurane and Aβ downregulated CA1-dendritic spines. Moreover, compared with either sevoflurane or Aβ alone, pre-treatment with Aβ isoforms followed by sevoflurane application in general further enhanced spine loss. This enhancement was related to MEGF10-related astrocyte-dependent synaptic engulfment, only in AβpE3 + 1.2 MAC sevoflurane and 3NTyrAβ + 1.2 MAC sevoflurane condition. In addition, removal of sevoflurane alleviated spine loss in Aβ + sevoflurane. In summary, these results suggest that both synapses and astrocytes are sensitive targets for sevoflurane; in the presence of 3NTyrAβ, 1.2 MAC sevoflurane alleviated astrocyte-mediated synaptic engulfment and exerted a lasting effect on dendritic spine remodeling.
Keywords: anesthesia; Alzheimer’s disease; spine dynamics; synaptic plasticity; astrocyte-mediated synaptic phagocytosis anesthesia; Alzheimer’s disease; spine dynamics; synaptic plasticity; astrocyte-mediated synaptic phagocytosis

Share and Cite

MDPI and ACS Style

Shi, Q.; Wang, X.; Pradhan, A.K.; Fenzl, T.; Rammes, G. The Effects of Sevoflurane and Aβ Interaction on CA1 Dendritic Spine Dynamics and MEGF10-Related Astrocytic Synapse Engulfment. Int. J. Mol. Sci. 2024, 25, 7393. https://doi.org/10.3390/ijms25137393

AMA Style

Shi Q, Wang X, Pradhan AK, Fenzl T, Rammes G. The Effects of Sevoflurane and Aβ Interaction on CA1 Dendritic Spine Dynamics and MEGF10-Related Astrocytic Synapse Engulfment. International Journal of Molecular Sciences. 2024; 25(13):7393. https://doi.org/10.3390/ijms25137393

Chicago/Turabian Style

Shi, Qinfang, Xingxing Wang, Arpit Kumar Pradhan, Thomas Fenzl, and Gerhard Rammes. 2024. "The Effects of Sevoflurane and Aβ Interaction on CA1 Dendritic Spine Dynamics and MEGF10-Related Astrocytic Synapse Engulfment" International Journal of Molecular Sciences 25, no. 13: 7393. https://doi.org/10.3390/ijms25137393

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop