The Effects of Sevoflurane and Aβ Interaction on CA1 Dendritic Spine Dynamics and MEGF10-Related Astrocytic Synapse Engulfment
Abstract
:1. Introduction
2. Results
2.1. Effects of Sevoflurane and Its Interaction with Aβ Isoforms on DSD
2.1.1. Sevoflurane at 0.4/1.2 MAC Decreases DSD
2.1.2. Effects of Aβ Pre-Treatment 90 min + Sevoflurane 90 min on DSD
2.1.3. Effects of Sevoflurane Removal for 90 min on DSD
2.2. Effects of Sevoflurane and Its Interaction with Aβ on Astrocyte-Mediated Synaptic Engulfment
2.2.1. Effects of Aβ Pre-Treatment 90 min + Sevoflurane 90 min on Astrocyte-Mediated Synaptic Engulfment
2.2.2. 1.2 MAC Sevoflurane Decreases Astrocyte-Mediated Synaptic Engulfment in Acute Hippocampal Brain Slices Pretreated with AβpE3/3NTyrAβ
2.2.3. Removal of 1.2 MAC Sevoflurane Decreases Astrocyte-Mediated Synaptic Engulfment in Acute Hippocampal Brain Slices Pretreated Only with 3NTyrAβ
2.3. Effects of Sevoflurane and Its Interaction with 3NTyrAβ on the Expression of MEGF10
2.3.1. Neither 0.4 MAC nor 1.2 MAC Sevoflurane Affects the MEGF10 Expressions
2.3.2. The Downregulation of MEGF10 Expression by Co-Application of 1.2 MAC Sevoflurane with 3NTyrAβ Is Diminished after the Removal of Sevoflurane
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Preparation of Acute Hippocampal Brain Slices
4.3. Aβ Preparation and Incubation
4.4. Sevoflurane Application
4.5. Experiment Schedule and Workflow
4.6. Analysis of Dendritic Spines
4.7. Immunofluorescence and Colocalization Analysis
4.7.1. Immunofluorescence Staining and Image Acquisition
4.7.2. Quantitative Analysis of Astrocyte-Mediated Synaptic Engulfment
4.8. Western Blotting
4.8.1. Sample Preparation
4.8.2. Preparation of Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)
4.8.3. Electrophoresis and Transferring
4.8.4. Blocking and Antibody Incubation
4.8.5. Analysis of Western Blots
4.9. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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DSD | Aβ1-40 | Aβ1-42 | AβpE3 | 3NTyrAβ | Compared to? | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Thin | Stubby | Mushroom | Thin | Stubby | Mushroom | Thin | Stubby | Mushroom | Thin | Stubby | Mushroom | ||
0.4 MAC Sevo | — | — | — | — | — | control | |||||||
p = 0.0008 | 0.035 | 0.156 | 0.0006 | 0.040 | 0.768 | 0.012 | 0.154 | 0.610 | <0.0001 | 0.014 | 0.082 | ||
— | — | — | — | — | — | — | — | — | Aβ 180 min | ||||
0.542 | 0.041 | 0.809 | 0.976 | 0.007 | 0.987 | 0.714 | 0.040 | 0.974 | 0.960 | 0.115 | 0.677 | ||
1.2 MAC Sevo | — | control | |||||||||||
0.0002 | 0.019 | 0.014 | 0.027 | 0.043 | 0.044 | 0.035 | 0.160 | 0.051 | 0.005 | 0.006 | 0.009 | ||
— | — | — | — | — | Aβ 180 min | ||||||||
0.885 | 0.025 | 0.023 | 0.698 | 0.005 | 0.150 | 0.783 | 0.035 | 0.055 | 0.033 | 0.044 | 0.074 |
DSD | Aβ1-40 | Aβ1-42 | AβpE3 | 3NTyrAβ | Compared to? | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Thin | Stubby | Mushroom | Thin | Stubby | Mushroom | Thin | Stubby | Mushroom | Thin | Stubby | Mushroom | ||
0.4 MAC Sevo washout | — | — | — | control | |||||||||
p < 0.0001 | 0.003 | 0.053 | 0.0005 | 0.025 | 0.105 | 0.037 | 0.003 | 0.146 | <0.0001 | 0.002 | 0.062 | ||
— | — | — | — | — | — | — | — | — | — | — | — | Aβ 270 min | |
0.618 | 0.994 | 0.347 | 0.629 | 0.672 | 0.887 | 0.995 | 0.637 | 0.758 | 1.000 | 0.185 | 0.755 | ||
1.2 MAC Sevo washout | — | — | — | control | |||||||||
0.003 | 0.003 | 0.110 | <0.0001 | 0.002 | 0.052 | 0.028 | 0.776 | 0.023 | 0.403 | 0.033 | 0.015 | ||
— | — | — | — | — | — | — | — | — | — | Aβ 270 min | |||
0.656 | 0.806 | 0.193 | 0.296 | 0.156 | 0.804 | 0.997 | 0.035 | 0.082 | 0.007 | 1.000 | 0.844 |
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Shi, Q.; Wang, X.; Pradhan, A.K.; Fenzl, T.; Rammes, G. The Effects of Sevoflurane and Aβ Interaction on CA1 Dendritic Spine Dynamics and MEGF10-Related Astrocytic Synapse Engulfment. Int. J. Mol. Sci. 2024, 25, 7393. https://doi.org/10.3390/ijms25137393
Shi Q, Wang X, Pradhan AK, Fenzl T, Rammes G. The Effects of Sevoflurane and Aβ Interaction on CA1 Dendritic Spine Dynamics and MEGF10-Related Astrocytic Synapse Engulfment. International Journal of Molecular Sciences. 2024; 25(13):7393. https://doi.org/10.3390/ijms25137393
Chicago/Turabian StyleShi, Qinfang, Xingxing Wang, Arpit Kumar Pradhan, Thomas Fenzl, and Gerhard Rammes. 2024. "The Effects of Sevoflurane and Aβ Interaction on CA1 Dendritic Spine Dynamics and MEGF10-Related Astrocytic Synapse Engulfment" International Journal of Molecular Sciences 25, no. 13: 7393. https://doi.org/10.3390/ijms25137393