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Open AccessArticle
PpGATA21 Enhances the Expression of PpGA2ox7 to Regulate the Mechanism of Cerasus humilis Rootstock-Mediated Dwarf in Peach Trees
by
Xiuzhen Li
Xiuzhen Li 1,
Ruxin Wang
Ruxin Wang 1,2,
Yuman Wang
Yuman Wang 1,
Xueqiang Li
Xueqiang Li 1,
Qiaofang Shi
Qiaofang Shi 1,2 and
Yihe Yu
Yihe Yu 1,2,*
1
College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, China
2
Henan Provincial Engineering Research Center on Characteristic Berry Germplasm Innovation & Utilization, Luoyang 471023, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(13), 7402; https://doi.org/10.3390/ijms25137402 (registering DOI)
Submission received: 3 June 2024
/
Revised: 28 June 2024
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Accepted: 4 July 2024
/
Published: 5 July 2024
Abstract
Dwarfing rootstocks enhance planting density, lower tree height, and reduce both labor in peach production. Cerasus humilis is distinguished by its dwarf stature, rapid growth, and robust fruiting capabilities, presenting substantial potential for further development. In this study, Ruipan 4 was used as the scion and grafted onto Amygdalus persica and Cerasus humilis, respectively. The results indicate that compared to grafting combination R/M (Ruipan 4/Amygdalus persica), grafting combination R/O (Ruipan 4/Cerasus humilis) plants show a significant reduction in height and a significant increase in flower buds. RNA-seq indicates that genes related to gibberellin (GA) and auxin metabolism are involved in the dwarfing process of scions mediated by C. humilis. The expression levels of the GA metabolism-related gene PpGA2ox7 significantly increased in R/O and are strongly correlated with plant height, branch length, and internode length. Furthermore, GA levels were significantly reduced in R/O. The transcription factor PpGATA21 was identified through yeast one-hybrid screening of the PpGA2ox7 promoter. Yeast one-hybrid (Y1H) and dual-luciferase reporter (DLR) demonstrate that PpGATA21 can bind to the promoter of PpGA2ox7 and activate its expression. Overall, PpGATA21 activates the expression of the GA-related gene PpGA2ox7, resulting in reduced GA levels and consequent dwarfing of plants mediated by C. humilis. This study provides new insights into the mechanisms of C. humilis and offers a scientific foundation for the dwarfing and high-density cultivation of peach trees.
Share and Cite
MDPI and ACS Style
Li, X.; Wang, R.; Wang, Y.; Li, X.; Shi, Q.; Yu, Y.
PpGATA21 Enhances the Expression of PpGA2ox7 to Regulate the Mechanism of Cerasus humilis Rootstock-Mediated Dwarf in Peach Trees. Int. J. Mol. Sci. 2024, 25, 7402.
https://doi.org/10.3390/ijms25137402
AMA Style
Li X, Wang R, Wang Y, Li X, Shi Q, Yu Y.
PpGATA21 Enhances the Expression of PpGA2ox7 to Regulate the Mechanism of Cerasus humilis Rootstock-Mediated Dwarf in Peach Trees. International Journal of Molecular Sciences. 2024; 25(13):7402.
https://doi.org/10.3390/ijms25137402
Chicago/Turabian Style
Li, Xiuzhen, Ruxin Wang, Yuman Wang, Xueqiang Li, Qiaofang Shi, and Yihe Yu.
2024. "PpGATA21 Enhances the Expression of PpGA2ox7 to Regulate the Mechanism of Cerasus humilis Rootstock-Mediated Dwarf in Peach Trees" International Journal of Molecular Sciences 25, no. 13: 7402.
https://doi.org/10.3390/ijms25137402
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