PpGATA21 Enhances the Expression of PpGA2ox7 to Regulate the Mechanism of Cerasus humilis Rootstock-Mediated Dwarf in Peach Trees
Abstract
:1. Introduction
2. Result
2.1. C. humilis Significantly Reduces the Height of Scions
2.2. Differentially Expressed Genes (DEGs) between R/O and R/M
2.3. Weighted Gene Co-Expression Network Analysis (WGCNA Analysis)
2.4. PpGA2ox7 Regulates GA Levels in Peach Trees
2.5. PpGATA21 Directly Binds and Activates PpGA2ox7 Expression
2.6. PpTATA21 Is a GATA-Type Transcriptional Activator
3. Discussion
4. Materials and Methods
4.1. Fruit Materials and Treatments
4.2. Measurement of Plant Growth Parameters
4.3. RNA Extraction and RNA-Seq Analysis
4.4. RT-qPCR
4.5. Quantification of Endogenous Hormones
4.6. Quantitative GUS Assay
4.7. Yeast One-Hybrid (Y1H)
4.8. Transcriptional Activation Analysis in Yeast Cells
4.9. Subcellular Localization Analysis
4.10. Dual-Luciferase Reporter (DLR)
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Li, X.; Wang, R.; Wang, Y.; Li, X.; Shi, Q.; Yu, Y. PpGATA21 Enhances the Expression of PpGA2ox7 to Regulate the Mechanism of Cerasus humilis Rootstock-Mediated Dwarf in Peach Trees. Int. J. Mol. Sci. 2024, 25, 7402. https://doi.org/10.3390/ijms25137402
Li X, Wang R, Wang Y, Li X, Shi Q, Yu Y. PpGATA21 Enhances the Expression of PpGA2ox7 to Regulate the Mechanism of Cerasus humilis Rootstock-Mediated Dwarf in Peach Trees. International Journal of Molecular Sciences. 2024; 25(13):7402. https://doi.org/10.3390/ijms25137402
Chicago/Turabian StyleLi, Xiuzhen, Ruxin Wang, Yuman Wang, Xueqiang Li, Qiaofang Shi, and Yihe Yu. 2024. "PpGATA21 Enhances the Expression of PpGA2ox7 to Regulate the Mechanism of Cerasus humilis Rootstock-Mediated Dwarf in Peach Trees" International Journal of Molecular Sciences 25, no. 13: 7402. https://doi.org/10.3390/ijms25137402