Identifying Diagnostic Markers and Constructing Predictive Models for Oxidative Stress in Multiple Sclerosis
Abstract
:1. Introduction
2. Result
2.1. Identification of DE-OSRGs in Multiple Sclerosis
2.2. Functional Enrichment Analysis of DE-OSRGs in Multiple Sclerosis
2.3. Identification of Diagnostic Genes for Multiple Sclerosis
2.4. Establishment and Evaluation of Multiple Sclerosis Diagnostic Prediction Model
2.5. Expressions of the Four Characteristic Genes in the EAE Model
2.6. Association between Characteristic Genes and the Proportion of Infiltrating Immune Cells
2.7. Feature Genes and Small Molecular Compound Docking
3. Discussion
4. Materials and Methods
4.1. Data Sources
4.2. Identification of Differentially Expressed Genes (DEGs)
4.3. Pathway Enrichment Analysis
4.4. Optimal Diagnostic Biomarker Selection and Model Building
4.5. Immune Infiltration Analysis
4.6. EAE Model and Scoring
4.7. Chemical Index Detection
4.8. RNA Extraction and qRT-PCR
4.9. H&E Staining
4.10. Flow Cytometry Analysis
4.11. Drugs Screened and Docking
4.12. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ma, Y.; Wang, F.; Zhao, Q.; Zhang, L.; Chen, S.; Wang, S. Identifying Diagnostic Markers and Constructing Predictive Models for Oxidative Stress in Multiple Sclerosis. Int. J. Mol. Sci. 2024, 25, 7551. https://doi.org/10.3390/ijms25147551
Ma Y, Wang F, Zhao Q, Zhang L, Chen S, Wang S. Identifying Diagnostic Markers and Constructing Predictive Models for Oxidative Stress in Multiple Sclerosis. International Journal of Molecular Sciences. 2024; 25(14):7551. https://doi.org/10.3390/ijms25147551
Chicago/Turabian StyleMa, Yantuanjin, Fang Wang, Qiting Zhao, Lili Zhang, Shunmei Chen, and Shufen Wang. 2024. "Identifying Diagnostic Markers and Constructing Predictive Models for Oxidative Stress in Multiple Sclerosis" International Journal of Molecular Sciences 25, no. 14: 7551. https://doi.org/10.3390/ijms25147551