Eicosapentaenoic Acid Rescues Cav1.2-L-Type Ca2+ Channel Decline Caused by Saturated Fatty Acids via Both Free Fatty Acid Receptor 4-Dependent and -Independent Pathways in Cardiomyocytes
Abstract
:1. Introduction
2. Results
2.1. Actions of Saturated Fatty Acid on the L-Type Ca2+ Channel and the Cellular Excitability
2.2. EPA Rescued OAPA-Induced Reduction in L-Type Ca2+ Channel
2.3. Actions of FFAR4 in Cardiomyocytes
2.4. Actions of ROS for Cav1.2 Expression
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Isolation of Neonatal Mouse Cardiomyocytes
4.3. Preparation of OAPA and Cell Culture
4.4. Measurement of Intracellular ROS Accumulation
4.5. Electrophysiological Measurements
4.6. Quantitative Real-Time PCR
4.7. Western Blot Analysis
4.8. Immunocytochemistry
4.9. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Morishima, M.; Wang, P.; Horii, K.; Horikawa, K.; Ono, K. Eicosapentaenoic Acid Rescues Cav1.2-L-Type Ca2+ Channel Decline Caused by Saturated Fatty Acids via Both Free Fatty Acid Receptor 4-Dependent and -Independent Pathways in Cardiomyocytes. Int. J. Mol. Sci. 2024, 25, 7570. https://doi.org/10.3390/ijms25147570
Morishima M, Wang P, Horii K, Horikawa K, Ono K. Eicosapentaenoic Acid Rescues Cav1.2-L-Type Ca2+ Channel Decline Caused by Saturated Fatty Acids via Both Free Fatty Acid Receptor 4-Dependent and -Independent Pathways in Cardiomyocytes. International Journal of Molecular Sciences. 2024; 25(14):7570. https://doi.org/10.3390/ijms25147570
Chicago/Turabian StyleMorishima, Masaki, Pu Wang, Kosuke Horii, Kazuki Horikawa, and Katsushige Ono. 2024. "Eicosapentaenoic Acid Rescues Cav1.2-L-Type Ca2+ Channel Decline Caused by Saturated Fatty Acids via Both Free Fatty Acid Receptor 4-Dependent and -Independent Pathways in Cardiomyocytes" International Journal of Molecular Sciences 25, no. 14: 7570. https://doi.org/10.3390/ijms25147570