Next Article in Journal
Feed Components and Timing to Improve the Feed Conversion Ratio for Sustainable Aquaculture Using Starch
Previous Article in Journal
Metabolic Shift in Porcine Spermatozoa during Sperm Capacitation-Induced Zinc Flux
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Genome-Wide Association Studies on Resistance to Pea Weevil: Identification of Novel Sources of Resistance and Associated Markers

Institute for Sustainable Agriculture, Spanish National Research Council (CSIC), Av. Menéndez Pidal s/n, 14004 Córdoba, Spain
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(14), 7920; https://doi.org/10.3390/ijms25147920
Submission received: 24 June 2024 / Revised: 16 July 2024 / Accepted: 17 July 2024 / Published: 19 July 2024
(This article belongs to the Section Molecular Genetics and Genomics)

Abstract

Little resistance to the pea weevil insect pest (Bruchus pisorum) is available in pea (Pisum sativum) cultivars, highlighting the need to search for sources of resistance in Pisum germplasm and to decipher the genetic basis of resistance. To address this need, we screened the response to pea weevil in a Pisum germplasm collection (324 accession, previously genotyped) under field conditions over four environments. Significant variation for weevil seed infestation (SI) was identified, with resistance being frequent in P. fulvum, followed by P. sativum ssp. elatius, P. abyssinicum, and P. sativum ssp. humile. SI tended to be higher in accessions with lighter seed color. SI was also affected by environmental factors, being favored by high humidity during flowering and hampered by warm winter temperatures and high evapotranspiration during and after flowering. Merging the phenotypic and genotypic data allowed genome-wide association studies (GWAS) yielding 73 markers significantly associated with SI. Through the GWAS models, 23 candidate genes were found associated with weevil resistance, highlighting the interest of five genes located on chromosome 6. These included gene 127136761 encoding squalene epoxidase; gene 127091639 encoding a transcription factor MYB SRM1; gene 127097033 encoding a 60S ribosomal protein L14; gene 127092211, encoding a BolA-like family protein, which, interestingly, was located within QTL BpLD.I, earlier described as conferring resistance to weevil in pea; and gene 127096593 encoding a methyltransferase. These associated genes offer valuable potential for developing pea varieties resistant to Bruchus spp. and efficient utilization of genomic resources through marker-assisted selection (MAS).
Keywords: Bruchus; GWAS; Pisum; resistance breeding Bruchus; GWAS; Pisum; resistance breeding

Share and Cite

MDPI and ACS Style

Osuna-Caballero, S.; Cobos, M.J.; Ruiz, C.M.; Wohor, O.Z.; Rispail, N.; Rubiales, D. Genome-Wide Association Studies on Resistance to Pea Weevil: Identification of Novel Sources of Resistance and Associated Markers. Int. J. Mol. Sci. 2024, 25, 7920. https://doi.org/10.3390/ijms25147920

AMA Style

Osuna-Caballero S, Cobos MJ, Ruiz CM, Wohor OZ, Rispail N, Rubiales D. Genome-Wide Association Studies on Resistance to Pea Weevil: Identification of Novel Sources of Resistance and Associated Markers. International Journal of Molecular Sciences. 2024; 25(14):7920. https://doi.org/10.3390/ijms25147920

Chicago/Turabian Style

Osuna-Caballero, Salvador, María J. Cobos, Carmen M. Ruiz, Osman Z. Wohor, Nicolas Rispail, and Diego Rubiales. 2024. "Genome-Wide Association Studies on Resistance to Pea Weevil: Identification of Novel Sources of Resistance and Associated Markers" International Journal of Molecular Sciences 25, no. 14: 7920. https://doi.org/10.3390/ijms25147920

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop