Identification of the Key Gene DfCCoAOMT1 through Comparative Analysis of Lignification in Dendrocalamus farinosus XK4 and ZPX Bamboo Shoots during Cold Storage
Abstract
:1. Introduction
2. Results
2.1. Analysis of the Main Nutritional Composition of ZPX and XK4 D. farinosus Bamboo Shoots
2.2. Changes in Lignin Content of Bamboo Shoots ZPX and XK4 under Cold Storage Conditions
2.3. Transcriptome Sequencing Reveals Differential Gene Expression in Cold-Stored D. farinosus Bamboo Shoots ZPX and XK4
2.4. WGCNA Analysis of DEGs of ZPX and XK4 Bamboo Shoots after Cold Storage for 1d and 5d
2.5. Expression Levels of Lignin Synthase Genes in Two Bamboo Shoots under Cold Storage Conditions
2.6. Heterologous Overexpression of DfCCoAOMT1 in Tobacco Significantly Enhances Lignin Synthesis
3. Discussion
4. Materials and Methods
4.1. Plant Material and Cold Storage Treatments
4.2. RNA Extraction and RT-qPCR
4.3. RNA Sequencing
4.4. Weighted Correlation Network Analysis
4.5. Overexpression of DfCCoAOMT1 in Tobacco
4.6. Lignin Content Analysis
4.7. Histochemical Staining
4.8. Determination of Lignin Autofluorescence
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhao, X.; Song, W.; Chen, S.; Xu, G.; Long, Z.; Yang, H.; Cao, Y.; Hu, S. Identification of the Key Gene DfCCoAOMT1 through Comparative Analysis of Lignification in Dendrocalamus farinosus XK4 and ZPX Bamboo Shoots during Cold Storage. Int. J. Mol. Sci. 2024, 25, 8065. https://doi.org/10.3390/ijms25158065
Zhao X, Song W, Chen S, Xu G, Long Z, Yang H, Cao Y, Hu S. Identification of the Key Gene DfCCoAOMT1 through Comparative Analysis of Lignification in Dendrocalamus farinosus XK4 and ZPX Bamboo Shoots during Cold Storage. International Journal of Molecular Sciences. 2024; 25(15):8065. https://doi.org/10.3390/ijms25158065
Chicago/Turabian StyleZhao, Xin, Wenjuan Song, Sen Chen, Gang Xu, Zhijian Long, Heyi Yang, Ying Cao, and Shanglian Hu. 2024. "Identification of the Key Gene DfCCoAOMT1 through Comparative Analysis of Lignification in Dendrocalamus farinosus XK4 and ZPX Bamboo Shoots during Cold Storage" International Journal of Molecular Sciences 25, no. 15: 8065. https://doi.org/10.3390/ijms25158065