Sirtuin 5 (SIRT5) Suppresses Tumor Growth by Regulating Mitochondrial Metabolism and Synaptic Remodeling in Gliomas
Abstract
:1. Introduction
2. Results
2.1. SIRT5 Expression Correlates with Better Survival in Glioma Patients
2.2. SIRT5 Knockdown Promotes GBM Cell Growth In Vitro and In Vivo
2.3. SIRT5 Potentially Regulates GBM Cell Growth through Modulation of Mitochondrial Pathways
2.4. SIRT5 May Regulate Synapse Function and Immune Response in Gliomas
3. Discussion
4. Materials and Methods
4.1. Sample and Data Collection
4.2. Bulk Transcriptome Data Analysis
4.3. Single-Cell Transcriptome Data Analysis
4.4. Cell Culture
4.5. Transfection of Short Hairpin RNA (shRNA)
4.6. Real-Time Quantitative PCR (RT-qPCR)
4.7. Cell Proliferation Assay
4.8. Subcutaneous Xenograft Model
4.9. Statistical Analyses
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Tang, W.; Chen, B.; Leung, G.K.-K.; Kiang, K.M. Sirtuin 5 (SIRT5) Suppresses Tumor Growth by Regulating Mitochondrial Metabolism and Synaptic Remodeling in Gliomas. Int. J. Mol. Sci. 2024, 25, 9125. https://doi.org/10.3390/ijms25169125
Tang W, Chen B, Leung GK-K, Kiang KM. Sirtuin 5 (SIRT5) Suppresses Tumor Growth by Regulating Mitochondrial Metabolism and Synaptic Remodeling in Gliomas. International Journal of Molecular Sciences. 2024; 25(16):9125. https://doi.org/10.3390/ijms25169125
Chicago/Turabian StyleTang, Wanjun, Bo Chen, Gilberto Ka-Kit Leung, and Karrie M. Kiang. 2024. "Sirtuin 5 (SIRT5) Suppresses Tumor Growth by Regulating Mitochondrial Metabolism and Synaptic Remodeling in Gliomas" International Journal of Molecular Sciences 25, no. 16: 9125. https://doi.org/10.3390/ijms25169125