Rabies Virus Regulates Inflammatory Response in BV-2 Cells through Activation of Myd88 and NF-κB Signaling Pathways via TLR7
Abstract
:1. Introduction
2. Results
2.1. RABV Exhibiting Varying Virulence Levels Provoke an Upregulation in TLR7 in Mouse Brain Tissues
2.2. RABV Induces Chemokine Expression In Vitro in a TLR7-Dependent Manner
2.3. Activation of Myd88 Pathway Regulates RABV-Induced Cytokine Expression via TLR7 Signal
2.4. Activation of NF-κB Pathway Regulates RABV-Induced Cytokine Expression via TLR7 Signal
3. Discussion
4. Materials and Methods
4.1. Mouse Studies
4.2. Cell Culture and Reagents
4.3. Virus Strains
4.4. Viral Titration and Quantitative Real-Time PCR Assay (qRT-PCR)
4.5. Immunohistochemistry
4.6. ELISA
4.7. NF-κB Translocation
4.8. Western Blot
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Xie, Y.; Chi, Y.; Tao, X.; Yu, P.; Liu, Q.; Zhang, M.; Yang, N.; Liu, S.; Zhu, W. Rabies Virus Regulates Inflammatory Response in BV-2 Cells through Activation of Myd88 and NF-κB Signaling Pathways via TLR7. Int. J. Mol. Sci. 2024, 25, 9144. https://doi.org/10.3390/ijms25179144
Xie Y, Chi Y, Tao X, Yu P, Liu Q, Zhang M, Yang N, Liu S, Zhu W. Rabies Virus Regulates Inflammatory Response in BV-2 Cells through Activation of Myd88 and NF-κB Signaling Pathways via TLR7. International Journal of Molecular Sciences. 2024; 25(17):9144. https://doi.org/10.3390/ijms25179144
Chicago/Turabian StyleXie, Yuan, Yinglin Chi, Xiaoyan Tao, Pengcheng Yu, Qian Liu, Minghui Zhang, Nuo Yang, Shuqing Liu, and Wuyang Zhu. 2024. "Rabies Virus Regulates Inflammatory Response in BV-2 Cells through Activation of Myd88 and NF-κB Signaling Pathways via TLR7" International Journal of Molecular Sciences 25, no. 17: 9144. https://doi.org/10.3390/ijms25179144