Transcriptional Profiling of Staphylococcus aureus during the Transition from Asymptomatic Nasal Colonization to Skin Colonization/Infection in Patients with Atopic Dermatitis
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characteristics of Nasal and Skin Specimens from Patients with AD
2.2. In Vivo Transcriptional Profiling of S. aureus during Asymptomatic Nasal Colonization to Skin Colonization/Infection in Patients with AD
2.2.1. S. aureus Agr Activity Is Increased in the Skin Samples of Some Patients with AD
2.2.2. Gene Expression in the Nasal Cavity and Skin Environment Appears Similar
Global Transcriptional Regulators
Adhesins
Cell Wall Modification Enzymes
Immune Evasion
Proteases
Genes Involved in Iron Limitation and Cell Respiration
3. Materials and Methods
3.1. Ethics Statement
3.2. Study Population
3.3. Sampling, Bacteriological Analysis, and Growth of S. aureus Strains
3.4. RNA Isolation, Reverse Transcription, and Quantitative Real-Time PCR
3.5. Spa Genotyping
3.6. Data Visualization and Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Patient | Age | Sex | SCORAD | Comorbidities | S. aureus Isolate | Spa Typing ID | Deduced MLST CC | Accessory Gene Content | Phenotype |
---|---|---|---|---|---|---|---|---|---|
A | 19 | female | 77 | - | nose lesional skin non-lesional skin | t304 | - | sak, scn | hemolytic hemolytic hemolytic |
B | 61 | female | 72.6 | Asthma bronchiale Milk product allergy | nose lesional skin non-lesional skin | t1451 | - | chp, scn | hemolytic hemolytic hemolytic |
C | 39 | male | 80.4 | Asthma bronchiale Rhinoconjunctivitis | nose lesional skin non-lesional skin | t008 | CC8 | - | hemolytic hemolytic hemolytic |
D | 36 | male | 34.4 | Asthma bronchiale Rhinoconjunctivitis | nose lesional skin | t674 t1943 | - | sak, scn | hemolytic hemolytic |
E | 54 | male | 10.5 | Rhinoconjunctivitis | nose lesional skin non-lesional skin | t094 | - | chp, scn | hemolytic hemolytic hemolytic |
F | 38 | female | 47 | - | nose lesional skin 1 lesional skin 2 | t091 t091 t3697 | - | sak, scn | hemolytic hemolytic non-hemolytic |
G | 48 | male | 48 | Asthma bronchiale Rhinoconjunctivitis | nose lesional skin non-lesional skin | t190 | CC8 | sak, chp, scn | hemolytic hemolytic hemolytic |
H | 41 | male | 41.3 | Asthma bronchiale Contact allergy (Amerchol) | nose lesional skin non-lesional skin | t002 | CC5 | sak, chp, scn | hemolytic hemolytic hemolytic |
I | 53 | male | 48.6 | Contact allergy (Isoeugenol) | nose lesional skin | t026 | CC45 | sak, chp, scn | hemolytic hemolytic |
J | 19 | male | 45.5 | Asthma bronchiale Rhinoconjunctivitis | nose lesional skin non-lesional skin | t091 | - | sak, scn | hemolytic hemolytic hemolytic |
K | 38 | male | 38.5 | Asthma bronchiale Rhinoconjunctivitis Contact allergy | nose lesional skin non-lesional skin | t2953 | - | sak, chp, scn | hemolytic hemolytic hemolytic |
L | 35 | female | 29 | Asthma bronchiale Rhinoconjunctivitis | nose lesional skin | t091 t1268 | - | sak, scn sak, chp, scn | hemolytic hemolytic |
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Li, P.; Schulte, J.; Wurpts, G.; Hornef, M.W.; Wolz, C.; Yazdi, A.S.; Burian, M. Transcriptional Profiling of Staphylococcus aureus during the Transition from Asymptomatic Nasal Colonization to Skin Colonization/Infection in Patients with Atopic Dermatitis. Int. J. Mol. Sci. 2024, 25, 9165. https://doi.org/10.3390/ijms25179165
Li P, Schulte J, Wurpts G, Hornef MW, Wolz C, Yazdi AS, Burian M. Transcriptional Profiling of Staphylococcus aureus during the Transition from Asymptomatic Nasal Colonization to Skin Colonization/Infection in Patients with Atopic Dermatitis. International Journal of Molecular Sciences. 2024; 25(17):9165. https://doi.org/10.3390/ijms25179165
Chicago/Turabian StyleLi, Peijuan, Julia Schulte, Gerda Wurpts, Mathias W. Hornef, Christiane Wolz, Amir S. Yazdi, and Marc Burian. 2024. "Transcriptional Profiling of Staphylococcus aureus during the Transition from Asymptomatic Nasal Colonization to Skin Colonization/Infection in Patients with Atopic Dermatitis" International Journal of Molecular Sciences 25, no. 17: 9165. https://doi.org/10.3390/ijms25179165