miR156-SPL and miR169-NF-YA Modules Regulate the Induction of Somatic Embryogenesis in Arabidopsis via LEC- and Auxin-Related Pathways
Abstract
:1. Introduction
2. Results
2.1. Different MIRNA156 Genes Regulate SE Induction
2.2. miR156 Regulates SE Induction via Controlling SPLs
2.3. miR156 via SPL11 Might Regulate LEC Genes in SE
2.4. Different MIRNA169 Genes Contribute to miR169 Regulating SE Induction
2.5. miR169 Regulates Embryogenic Induction via Controlling NF-YA
NF-YA Control SE Induction
2.6. miR169 via NF-YA5 Regulates the Expression of Genes of Critical Role in SE
3. Discussion
3.1. miR156 through SPLs Controls SE Induction
3.2. Phytohormone and LEC Genes Related Mechanism of miR156-SPL Regulation in SE
3.3. Two miR169 Isoforms via NF-YA TFs Control SE Induction
3.4. miR169-NF-YA5 Module—A New Player in LEC1 and Auxin-Related Mechanisms Controlling SE Induction
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. Somatic Embryogenesis Induction In Vitro
4.3. Analysis of Target Gene Expression
4.4. GUS/GFP Signal Detection
4.5. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene/miRNA | Primer Sequence |
---|---|
SPL2 | [15] |
SPL3 | [15] |
SPL4 | F-GCGCTTAGCTGGACACAATG R-GTCTGGATCAGTTGACCGCT |
SPL5 | F-GCGGTCAACTGATCCAGACT R-AGAAGAGAGAGAGCGGGAGG |
SPL9 | [15] |
SPL10 | [15] |
SPL11 | F-GCAGGTTCCATGCTGTCTCT R-ACGACGCCTCGCATTATGAT |
SPL13 | [15] |
SPL15 | F-TAATGTGTTCGGGTCAGGCC R -TCCGGATCCATCCTCGAAGT |
LEC1 | [91] |
LEC2 | [91] |
FUS3 | [91] |
NF-YA5 | [15] |
YUC4 | [64] |
YUC10 | [64] |
PIN1 | F-AACCGTTTCGTCGCTCTCTT R-ACGGAGGTTCATGGCGTAAG |
miR156 | [15] |
miR169 | [15] |
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Nowak, K.; Wójcik, A.M.; Konopka, K.; Jarosz, A.; Dombert, K.; Gaj, M.D. miR156-SPL and miR169-NF-YA Modules Regulate the Induction of Somatic Embryogenesis in Arabidopsis via LEC- and Auxin-Related Pathways. Int. J. Mol. Sci. 2024, 25, 9217. https://doi.org/10.3390/ijms25179217
Nowak K, Wójcik AM, Konopka K, Jarosz A, Dombert K, Gaj MD. miR156-SPL and miR169-NF-YA Modules Regulate the Induction of Somatic Embryogenesis in Arabidopsis via LEC- and Auxin-Related Pathways. International Journal of Molecular Sciences. 2024; 25(17):9217. https://doi.org/10.3390/ijms25179217
Chicago/Turabian StyleNowak, Katarzyna, Anna M. Wójcik, Katarzyna Konopka, Alicja Jarosz, Katarzyna Dombert, and Małgorzata D. Gaj. 2024. "miR156-SPL and miR169-NF-YA Modules Regulate the Induction of Somatic Embryogenesis in Arabidopsis via LEC- and Auxin-Related Pathways" International Journal of Molecular Sciences 25, no. 17: 9217. https://doi.org/10.3390/ijms25179217