Effects of Dietary Bio-Fermented Selenium Supplementation on Growth, Immune Performance, and Intestinal Microflora of Chinese Mitten Crabs, Eriocheir sinensis
Abstract
:1. Introduction
2. Results
2.1. Growth Performance
2.2. Activities of Antioxidant Enzyme
2.3. Immunity-Related Genes Expression
2.4. Intestinal Morphology
2.5. Intestinal Flora and OTUs
2.6. Intestinal Microbial Diversity and Composition
3. Discussion
4. Materials and Methods
4.1. Diets Preparation
4.2. Experimental Animals and Management Procedure
4.3. Sample Collection
4.4. Growth Parameters Analysis
4.5. Biochemical Analysis
4.6. Quantitative Real-Time PCR Analysis (qRT-PCR)
4.7. Analysis of Intestinal Morphology
4.8. Analysis of Bacterial Diversity in the Intestine
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bio−Se (mg/kg) | Wt (g) | WGR (%) | SGR (%/day) | SR (%) |
---|---|---|---|---|
0 | 18.14 ± 0.34 c | 11.81 ± 0.27 d | 0.38 ± 0.03 bc | 63.33 ± 6.67 b |
0.3 | 18.47 ± 0.32 b | 12.54 ± 0.41 c | 0.42 ± 0.03 bc | 85.93 ± 2.97 a |
0.6 | 18.70 ± 0.49 ab | 13.71 ± 0.48 b | 0.45 ± 0.03 ab | 80.96 ± 0.94 a |
1.5 | 18.87 ± 0.45 a | 14.74 ± 0.48 a | 0.50 ± 0.03 a | 87.43 ± 1.73 a |
3.0 | 18.11 ± 0.32 c | 10.32 ± 0.13 d | 0.36 ± 0.04 c | 69.83 ± 0.17 b |
Group | Chao1 | Observed_Features | Pielou_e | Shannon | Simple | Coverage |
---|---|---|---|---|---|---|
CG | 362.04 ± 27.95 a | 317.06 ± 51.27 a | 0.63 ± 0.12 a | 4.78 ± 0.00 a | 0.91 ± 0.02 a | 0.999 ± 0.00 a |
Bio−Se | 552.06 ± 62.94 b | 438.17 ± 94.29 b | 0.59 ± 0.13 a | 4.77 ± 0.02 a | 0.90 ±0.02 a | 0.997 ± 0.01 a |
Taxonomy | Firmicutes | Proteobacteria | Bacteroidota | Fusobacteriota | Campilobacterota |
---|---|---|---|---|---|
CG | 54.62 | 23.66 | 15.47 | 0.45 | 0.16 |
Bio−Se | 49.81 | 25.02 | 11.77 | 9.80 | 1.32 |
Ingredient | (%) |
---|---|
Fish meal 1 | 35.0 |
Bean meal | 25.0 |
Peanut meal | 16.0 |
Corn starch | 5.7 |
Soybean oil 2 | 4.0 |
yeast | 5.0 |
Cellulose | 4.7 |
Vitamin mix 3 | 2.0 |
Se-free mineral mix 4 | 2.0 |
Cholesterol | 0.3 |
Choline chloride | 0.2 |
Ethopabate | 0.01 |
Calcium propionate | 0.10 |
Crude protein | 44.8 |
Crude lipid | 7.8 |
Primer | Sequence (5′-3′) |
---|---|
EsERK-F | TTCAGCAACAGGCTCATC |
EsERK-R | TGTTCAGGAGGAGGTTTGATGGC |
EsRelish-F | TCTCCCTACTCTGACCATTCC |
EsRelish-R | TTCCCACCATCTCACTCTTGT |
EsALF-1-F | GACGCAGGAGGATGCTAAC |
EsALF-1-R | TGATGGCAGATGAAGGACAC |
EsCrus-1-F | GCTCTATGGCGGAGGATGTCA |
EsCrus-1-R | CGGGCTTCAGACCCACTTTAC |
EsLYS-F | CTGGGATGATGTGGAGAAGTGC |
EsLYS-R | TTATTCGGTGTGTTATGAGGGGT |
Esβ-actin-F | GCATCCACGAGACCACTTACA |
Esβ-actin-R | CTCCTGCTTGCTGATCCACATC |
EsV3-V4-F EsV3-V4-R | CCTACGGGAGGCAGCAG GACTACCAGGGTATCTAATC |
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Yang, Z.; Li, J.; Ma, Y.; Wu, Z.; Li, J.; Wang, F.; Xi, Y.; Jiang, Y.; Huang, S.; Yi, Q. Effects of Dietary Bio-Fermented Selenium Supplementation on Growth, Immune Performance, and Intestinal Microflora of Chinese Mitten Crabs, Eriocheir sinensis. Int. J. Mol. Sci. 2024, 25, 9219. https://doi.org/10.3390/ijms25179219
Yang Z, Li J, Ma Y, Wu Z, Li J, Wang F, Xi Y, Jiang Y, Huang S, Yi Q. Effects of Dietary Bio-Fermented Selenium Supplementation on Growth, Immune Performance, and Intestinal Microflora of Chinese Mitten Crabs, Eriocheir sinensis. International Journal of Molecular Sciences. 2024; 25(17):9219. https://doi.org/10.3390/ijms25179219
Chicago/Turabian StyleYang, Zhichao, Jialin Li, Yuhan Ma, Zihao Wu, Jiaming Li, Fengchi Wang, Yuting Xi, Yusheng Jiang, Shu Huang, and Qilin Yi. 2024. "Effects of Dietary Bio-Fermented Selenium Supplementation on Growth, Immune Performance, and Intestinal Microflora of Chinese Mitten Crabs, Eriocheir sinensis" International Journal of Molecular Sciences 25, no. 17: 9219. https://doi.org/10.3390/ijms25179219