Genomic Dissection of an Enteroaggregative Escherichia coli Strain Isolated from Bacteremia Reveals Insights into Its Hybrid Pathogenic Potential
Abstract
:1. Introduction
2. Results
2.1. EC092 Draft Genome
2.2. EC092 Genetic Features Provide Insights into Its Virulence
2.3. Phylogenetic Analyses Show That EC092 Is Closely Related to Other EAEC Strains
2.4. Virulence Factors Distribution Shows Different Patterns in Various EAEC
2.5. Genetic Neighborhood Analysis Reveals a Close Relationship between the EC092 and EAEC Strains K44V1 and K45V1 Isolated from Feces
2.6. Pet, Pic, Sat, and SepA Production by EC092
2.7. E. coli EC092 Is Not Killed by Normal Human Serum (NHS)
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains
4.2. DNA Extraction, Library Sequencing, and Read Filtering
4.3. Assembly, Annotation, and Screening for Known Virulence Factors
4.4. Phylogenetic Relationships of EC092 with Different Groups of E. coli
4.5. Presence and Absence of Virulence Factors in Different EAEC Strains
4.6. Genomic Neighborhood
4.7. Detection of Pet, Pic, Sat, and SepA in the EC092 Culture Supernatant
4.8. Resistance against the Bactericidal Activity of Human Serum
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Groups a | Gene | Name |
---|---|---|
Virulence regulator | aggR | Transcriptional activator |
Adhesion and invasion | aap | Dispersin, anti-aggregation Protein |
aatABCDP | Dispersin transporter | |
Fimbriae | ecpABCDER | E. coli common pilus |
ycbFSTUV | Ycb fimbria | |
csgABCDEFG | Curli | |
Iron acquisition | entABCDEFS | Enterobactin |
fepABCDEG | Ferroenterobactin | |
fes | Ferric enterobactin esterase | |
fyuA | Siderophore receptor | |
irp2 | Yersiniabactin biosynthetic system | |
iucABCD | Aerobactin synthetase | |
iutA | Ferric aerobactin receptor | |
Type 2 secretion system | gspCDEFGHIJKLM | T2SS-1 |
Type 3 secretion system 2 | ygeGH | ETT2 |
eprHIJK | ETT2 | |
epaOPQRS | ETT2 | |
Type 6 secretion system | aaiABCDEFGHIJKLMNOPX | AggR-activated island |
Type 5 secretion system | pet | Pet |
pic | Pic | |
sat | Sat | |
sepA | SepA | |
upaG/ehaG | UPEC autotransporter | |
cah | Cah | |
ehaC | EHEC autotransporter | |
Toxins | hlyE | Hemolysin E |
Flagellum | flgEFGHIJKL | Flagellar protein |
fliAFGIJMPR | Flagellar protein | |
flhAB | Flagellar protein | |
Serum resistance | traT | Serum survival |
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Del Carpio, A.M.G.; Freire, C.A.; Andrade, F.B.; Piazza, R.M.F.; Silva, R.M.; Carvalho, E.; Elias, W.P. Genomic Dissection of an Enteroaggregative Escherichia coli Strain Isolated from Bacteremia Reveals Insights into Its Hybrid Pathogenic Potential. Int. J. Mol. Sci. 2024, 25, 9238. https://doi.org/10.3390/ijms25179238
Del Carpio AMG, Freire CA, Andrade FB, Piazza RMF, Silva RM, Carvalho E, Elias WP. Genomic Dissection of an Enteroaggregative Escherichia coli Strain Isolated from Bacteremia Reveals Insights into Its Hybrid Pathogenic Potential. International Journal of Molecular Sciences. 2024; 25(17):9238. https://doi.org/10.3390/ijms25179238
Chicago/Turabian StyleDel Carpio, Alejandra M. G., Claudia A. Freire, Fernanda B. Andrade, Roxane M. F. Piazza, Rosa M. Silva, Eneas Carvalho, and Waldir P. Elias. 2024. "Genomic Dissection of an Enteroaggregative Escherichia coli Strain Isolated from Bacteremia Reveals Insights into Its Hybrid Pathogenic Potential" International Journal of Molecular Sciences 25, no. 17: 9238. https://doi.org/10.3390/ijms25179238