Galectin-1 Attenuates PDGF-Mediated AKT Signaling in Retinal Pigment Epithelial Cells
Abstract
:1. Introduction
2. Results
2.1. Galectin-1 Colocalizes with PDGF-BB-Induced Clusters of PDGF-Rβ
2.2. PDGF-BB-Induced AKT and ERK1/2 Signaling Is Reduced by hr-Galectin-1
2.3. Galectin-1 Reduces PDGF-BB-Induced AKT Signaling in a Glycosylation-Dependent Manner
2.4. Lack of Endogenous Galectin-1 Enhances PDGF-BB-Mediated pAKT Signaling
2.5. Galectin-1 Reduces PDGF-BB-Mediated Clustering of PDGF-Rβ
2.6. Exogenous and Endogenous Galectin-1 Mitigates PDGF-BB-Mediated pAKT Signaling by Reducing PDGF-Rβ Expression in RPE Cells
2.7. Galectin-1 Attenuates PDGF-Induced RPE Cell Proliferation
3. Discussion
4. Methods
4.1. Cell Culture
4.2. Isolation of hr-Galectin-1
4.3. Cell Proliferation
4.4. Immunocytochemistry
4.5. Protein Preparation and Western Blot Analysis
4.6. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bizzotto, M.; Ostermaier, A.; Liesenhoff, C.; Ma, W.; Geerlof, A.; Priglinger, S.G.; Priglinger, C.S.; Ohlmann, A. Galectin-1 Attenuates PDGF-Mediated AKT Signaling in Retinal Pigment Epithelial Cells. Int. J. Mol. Sci. 2024, 25, 9267. https://doi.org/10.3390/ijms25179267
Bizzotto M, Ostermaier A, Liesenhoff C, Ma W, Geerlof A, Priglinger SG, Priglinger CS, Ohlmann A. Galectin-1 Attenuates PDGF-Mediated AKT Signaling in Retinal Pigment Epithelial Cells. International Journal of Molecular Sciences. 2024; 25(17):9267. https://doi.org/10.3390/ijms25179267
Chicago/Turabian StyleBizzotto, Martina, Annabella Ostermaier, Caspar Liesenhoff, Wenxiu Ma, Arie Geerlof, Siegfried G. Priglinger, Claudia S. Priglinger, and Andreas Ohlmann. 2024. "Galectin-1 Attenuates PDGF-Mediated AKT Signaling in Retinal Pigment Epithelial Cells" International Journal of Molecular Sciences 25, no. 17: 9267. https://doi.org/10.3390/ijms25179267