Isopropyl 3-(3,4-Dihydroxyphenyl)-2-hydroxypropanoate Alleviates Palmitic Acid-Induced Vascular Aging in HUVEC Cells through ROS/Ferroptosis Pathway
Abstract
:1. Introduction
2. Results
2.1. Experimental Results of MTT
2.2. IDHP Delays PA-Induced HUVEC Cell Senescence
2.3. Cellular ROS Experimental Results
2.4. GSH Detection
2.5. Fe2+ Detection
2.6. Detection of Lipid ROS
2.7. Flow Cytometry to Detect Cell Proliferation Rate
2.8. Western Blot
3. Discussion
4. Materials and Methods
4.1. Cell Line and Culture
4.2. Cell Viability—MTT Assay
4.3. Senescence-Associated β-Galactosidase Staining Assay
4.4. Cellular ROS Assay—DCFH-DA Probe
4.5. Lipid ROS Detection
4.6. Fe2+ Detection
4.7. GSH Detection
4.8. Flow Cytometry
4.9. Western Blot
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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He, X.; Zheng, X.; Xie, W. Isopropyl 3-(3,4-Dihydroxyphenyl)-2-hydroxypropanoate Alleviates Palmitic Acid-Induced Vascular Aging in HUVEC Cells through ROS/Ferroptosis Pathway. Int. J. Mol. Sci. 2024, 25, 9278. https://doi.org/10.3390/ijms25179278
He X, Zheng X, Xie W. Isopropyl 3-(3,4-Dihydroxyphenyl)-2-hydroxypropanoate Alleviates Palmitic Acid-Induced Vascular Aging in HUVEC Cells through ROS/Ferroptosis Pathway. International Journal of Molecular Sciences. 2024; 25(17):9278. https://doi.org/10.3390/ijms25179278
Chicago/Turabian StyleHe, Xin, Xiaohui Zheng, and Weidong Xie. 2024. "Isopropyl 3-(3,4-Dihydroxyphenyl)-2-hydroxypropanoate Alleviates Palmitic Acid-Induced Vascular Aging in HUVEC Cells through ROS/Ferroptosis Pathway" International Journal of Molecular Sciences 25, no. 17: 9278. https://doi.org/10.3390/ijms25179278