Mitigating Cold Ischemic Injury: HTK, UW and IGL-2 Solution’s Role in Enhancing Antioxidant Defence and Reducing Inflammation in Steatotic Livers
Abstract
:1. Introduction
2. Results
3. Discussion
4. Material and Methods
4.1. Animals
4.2. Experimental Groups
4.3. Preservation Solutions
4.4. Biochemical Assays
4.5. Statistical Analysis
5. Conclusions
6. Future Perspectives
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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HTK | UW | IGL-2 | |
---|---|---|---|
pH | 7.02–7.2 | 7.4 | 7.4 |
Osmolarity, mOsm/L | 310 | 320 | 360 |
Viscosity, cP | 1.8 | 5.70 | 1.7 |
Colloids | – | 50 g/L HES | 5 g/L PEG |
Impermeants | |||
Lactobionate mmol/L: | – | 100 | 100 |
Mannitol mmol/L: | 30 | – | 60 |
Raffinose mmol/L: | 30 | – | |
Buffers | Histidine | Phosphate, Sulfate | HEPES, Histidine, Phosphate, Sulfate |
Electrolytes | |||
Calcium mmol/L: | 0.015 | – | – |
Chloride mmol/L: | 50 | – | – |
Magnesium mmol/L: | 4 | 5 | 5 |
Potassium mmol/L: | 10 | 120 | 25 |
Sodium mmol/L: | 15 | 25 | 125 |
Zinc mmol/L: | – | – | 0.091 |
Metabolic Precursors | α-ketoglutarate | Adenosine | Adenosine, sodium nitrite |
Antioxidants and Free-Radical Scavengers | Tryptophan | Allopurinol, glutathione | Glutathione |
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Bardallo, R.G.; Chullo, G.; Alva, N.; Rosello-Catafau, J.; Fundora-Suárez, Y.; Carbonell, T.; Panisello-Rosello, A. Mitigating Cold Ischemic Injury: HTK, UW and IGL-2 Solution’s Role in Enhancing Antioxidant Defence and Reducing Inflammation in Steatotic Livers. Int. J. Mol. Sci. 2024, 25, 9318. https://doi.org/10.3390/ijms25179318
Bardallo RG, Chullo G, Alva N, Rosello-Catafau J, Fundora-Suárez Y, Carbonell T, Panisello-Rosello A. Mitigating Cold Ischemic Injury: HTK, UW and IGL-2 Solution’s Role in Enhancing Antioxidant Defence and Reducing Inflammation in Steatotic Livers. International Journal of Molecular Sciences. 2024; 25(17):9318. https://doi.org/10.3390/ijms25179318
Chicago/Turabian StyleBardallo, Raquel G., Gabriela Chullo, Norma Alva, Joan Rosello-Catafau, Yiliam Fundora-Suárez, Teresa Carbonell, and Arnau Panisello-Rosello. 2024. "Mitigating Cold Ischemic Injury: HTK, UW and IGL-2 Solution’s Role in Enhancing Antioxidant Defence and Reducing Inflammation in Steatotic Livers" International Journal of Molecular Sciences 25, no. 17: 9318. https://doi.org/10.3390/ijms25179318