Disruption of Poly(ADP-ribosyl)ation Improves Plant Tolerance to Methyl Viologen-Mediated Oxidative Stress via Induction of ROS Scavenging Enzymes
Abstract
:1. Introduction
2. Results
2.1. RNAi Silencing as Well as Pharmacological Inhibition of PARP1 Induces Tolerance to MV—Mediated Stress in Nicotiana Benthamiana
2.2. MV Does Not Affect Gene Expression and Subcellular Localization of PARP1
2.3. MV Activates Poly(ADP-ribosyl)ation
2.4. The Role of PARP1 in Accumulation of Plant Hormones
2.5. RNAi Silencing and Pharmacological Inhibition of PARP1 Activates Expression of Genes Encoding Major ROS Scavenging Antioxidant Enzymes
3. Discussion
4. Materials and Methods
4.1. Virus-Induced Silencing of PARP1 Expression
4.2. MV Stress and 3AB Treatments
4.3. Chemiluminescence Assay for H2O2
4.4. Immunolabelling and Confocal Imaging Analysis
4.5. Real Time Quantitative RT-PCR (RT-qPCR)
4.6. Immunological Detection of Poly ADP-Ribose (PAR)
4.7. Measurement of Endogenous SA
4.8. Quantification of SOD and CAT Activities
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kalinina, N.O.; Spechenkova, N.; Ilina, I.; Samarskaya, V.O.; Bagdasarova, P.; Zavriev, S.K.; Love, A.J.; Taliansky, M. Disruption of Poly(ADP-ribosyl)ation Improves Plant Tolerance to Methyl Viologen-Mediated Oxidative Stress via Induction of ROS Scavenging Enzymes. Int. J. Mol. Sci. 2024, 25, 9367. https://doi.org/10.3390/ijms25179367
Kalinina NO, Spechenkova N, Ilina I, Samarskaya VO, Bagdasarova P, Zavriev SK, Love AJ, Taliansky M. Disruption of Poly(ADP-ribosyl)ation Improves Plant Tolerance to Methyl Viologen-Mediated Oxidative Stress via Induction of ROS Scavenging Enzymes. International Journal of Molecular Sciences. 2024; 25(17):9367. https://doi.org/10.3390/ijms25179367
Chicago/Turabian StyleKalinina, Natalia O., Nadezhda Spechenkova, Irina Ilina, Viktoriya O. Samarskaya, Polina Bagdasarova, Sergey K. Zavriev, Andrew J. Love, and Michael Taliansky. 2024. "Disruption of Poly(ADP-ribosyl)ation Improves Plant Tolerance to Methyl Viologen-Mediated Oxidative Stress via Induction of ROS Scavenging Enzymes" International Journal of Molecular Sciences 25, no. 17: 9367. https://doi.org/10.3390/ijms25179367