CAIP-Induced ROS Production Contributes to Sustaining Atherosclerotic Process Associated with Helicobacter cinaedi Infection through Macrophages and Endothelial Cells Activation
Abstract
:1. Introduction
2. Results
2.1. CAIP Increases the Expression of LOX-1 Receptor on the Surface of Macrophages and Endothelial Cells
2.2. The Heightened Expression of LOX-1 Is Facilitated by ROS Induced by CAIP
2.3. CAIP-Mediated ROS Production Plays a Crucial Role in the Formation of Foam Cells
2.4. The CAIP-Mediated Phosphorylation of MAPKs ERK1/2 and p38, and the Activation of NFkB, Are Dependent on ROS
2.5. CAIP-Triggered Cytokines Production Is Affected by ROS Inhibition
3. Discussion
4. Materials and Methods
4.1. Ethics Statement
4.2. Purification of the Cells and Treatments
4.3. Flow Cytometry
4.4. ROS Detection
4.5. Foam Cell Formation
4.6. Western Blot
4.7. Detection of TNF-α, IL-1β, IL-6, CCL-2, and IL-8 in Culture Supernatants
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Paolini, E.; Cozzi, S.; Codolo, G. CAIP-Induced ROS Production Contributes to Sustaining Atherosclerotic Process Associated with Helicobacter cinaedi Infection through Macrophages and Endothelial Cells Activation. Int. J. Mol. Sci. 2024, 25, 9377. https://doi.org/10.3390/ijms25179377
Paolini E, Cozzi S, Codolo G. CAIP-Induced ROS Production Contributes to Sustaining Atherosclerotic Process Associated with Helicobacter cinaedi Infection through Macrophages and Endothelial Cells Activation. International Journal of Molecular Sciences. 2024; 25(17):9377. https://doi.org/10.3390/ijms25179377
Chicago/Turabian StylePaolini, Erika, Stefano Cozzi, and Gaia Codolo. 2024. "CAIP-Induced ROS Production Contributes to Sustaining Atherosclerotic Process Associated with Helicobacter cinaedi Infection through Macrophages and Endothelial Cells Activation" International Journal of Molecular Sciences 25, no. 17: 9377. https://doi.org/10.3390/ijms25179377