Quercetin Mitigates Lysophosphatidylcholine (LPC)-Induced Neutrophil Extracellular Traps (NETs) Formation through Inhibiting the P2X7R/P38MAPK/NOX2 Pathway
Abstract
:1. Introduction
2. Result
2.1. LPC Induces NETs Formation in Primary Rat Neutrophils
2.2. Quercetin Inhibits LPC-Induced NETs Formation
2.3. LPC-Induced NETs Formation Is Mediated by P2X7R Up-Regulation
2.4. LPC-Induced NETs Formation Is Associated with the P2X7R/P38MAPK/NOX2 Pathway
2.5. Quercetin Inhibits LPC-Induced NETs Formation by Regulating P2X7R
2.6. Quercetin Inhibits LPC-Induced NETs Formation by Regulating the P2X7R/P38MAPK/NOX2 Pathway
3. Discussion
4. Materials and Methods
4.1. Reagents and Antibodies
4.2. Animals
4.3. Primary Rat Neutrophils Isolation
4.4. Cell Culture and Treatment
4.5. Cell Viability Assays
4.6. Live-Cell Imaging
4.7. Immunofluorescence
4.8. Quantification of cf-DNA
4.9. MPO-DNA Complexes ELISA
4.10. Western Blot Analysis
4.11. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, S.; Wang, Y.; Ying, L.; Li, H.; Zhang, K.; Liang, N.; Luo, G.; Xiao, L. Quercetin Mitigates Lysophosphatidylcholine (LPC)-Induced Neutrophil Extracellular Traps (NETs) Formation through Inhibiting the P2X7R/P38MAPK/NOX2 Pathway. Int. J. Mol. Sci. 2024, 25, 9411. https://doi.org/10.3390/ijms25179411
Liu S, Wang Y, Ying L, Li H, Zhang K, Liang N, Luo G, Xiao L. Quercetin Mitigates Lysophosphatidylcholine (LPC)-Induced Neutrophil Extracellular Traps (NETs) Formation through Inhibiting the P2X7R/P38MAPK/NOX2 Pathway. International Journal of Molecular Sciences. 2024; 25(17):9411. https://doi.org/10.3390/ijms25179411
Chicago/Turabian StyleLiu, Si, Yan Wang, Linyao Ying, Hao Li, Keyi Zhang, Na Liang, Gang Luo, and Lin Xiao. 2024. "Quercetin Mitigates Lysophosphatidylcholine (LPC)-Induced Neutrophil Extracellular Traps (NETs) Formation through Inhibiting the P2X7R/P38MAPK/NOX2 Pathway" International Journal of Molecular Sciences 25, no. 17: 9411. https://doi.org/10.3390/ijms25179411