Antennal Transcriptome Screening and Identification of Chemosensory Proteins in the Double-Spine European Spruce Bark Beetle, Ips duplicatus (Coleoptera: Scolytinae)
Abstract
:1. Introduction
2. Results
2.1. De Novo Antennal Transcriptome Sequencing and Assembly
2.2. Odorant Receptors in I. duplicatus
2.3. Ionotropic Receptors and iGulR Family Receptors in I. duplicatus
2.4. Gustatory Receptors in I. duplicatus
2.5. Odorant-Binding Proteins in I. duplicatus
2.6. Chemosensory Proteins (CSPs) in I. duplicatus
2.7. Sensory Neuron Membrane Proteins (SNMPs) in I. duplicatus
3. Discussion
4. Materials and Methods
4.1. Insect Collection and Antennal Tissue Dissection
4.2. RNA Extraction and Sequencing
4.3. Transcriptome Assembly and Gene Annotation
4.4. Phylogenetic Analysis of Candidate Chemosensory Proteins
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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IDUP_AM1 | IDUP_AM2 | IDUP_AF1 | IDUP_AF2 | All Combined | |
---|---|---|---|---|---|
Total raw reads | 40,749,080 | 47,677,670 | 39,480,112 | 40,689,528 | |
Total transcripts | 105,416 | 121,285 | 91,822 | 98,264 | 204,588 |
Total genes | 61,659 | 70,337 | 50,264 | 54,391 | 125,878 |
GC content | 39.89 | 39.82 | 38.96 | 39.26 | 39.21 |
N50 length | 2101 | 2027 | 2455 | 2036 | 2317 |
Average length | 1049.33 | 1011.11 | 1244.16 | 1048.26 | 1027.03 |
Complete BUSCOs (insecta_odb10) | 93.71% | 94.37% | 95.17% | 94% | 99.71% |
BUSCOs fragmented % | 4.24 | 3.58 | 3.22 | 2.37 | 0.15 |
% mapped to genome * | 88.56% | 88.30% | 87.97% | 88.63% |
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Johny, J.; Große-Wilde, E.; Kalinová, B.; Roy, A. Antennal Transcriptome Screening and Identification of Chemosensory Proteins in the Double-Spine European Spruce Bark Beetle, Ips duplicatus (Coleoptera: Scolytinae). Int. J. Mol. Sci. 2024, 25, 9513. https://doi.org/10.3390/ijms25179513
Johny J, Große-Wilde E, Kalinová B, Roy A. Antennal Transcriptome Screening and Identification of Chemosensory Proteins in the Double-Spine European Spruce Bark Beetle, Ips duplicatus (Coleoptera: Scolytinae). International Journal of Molecular Sciences. 2024; 25(17):9513. https://doi.org/10.3390/ijms25179513
Chicago/Turabian StyleJohny, Jibin, Ewald Große-Wilde, Blanka Kalinová, and Amit Roy. 2024. "Antennal Transcriptome Screening and Identification of Chemosensory Proteins in the Double-Spine European Spruce Bark Beetle, Ips duplicatus (Coleoptera: Scolytinae)" International Journal of Molecular Sciences 25, no. 17: 9513. https://doi.org/10.3390/ijms25179513