R2R3 MYB Transcription Factor GhMYB201 Promotes Cotton Fiber Elongation via Cell Wall Loosening and Very-Long-Chain Fatty Acid Synthesis
Abstract
:1. Introduction
2. Results
2.1. GhMYB201 Is a Transcriptional Activator Preferentially Expressed in Elongating Fibers
2.2. GhMYB201 Knockout Negatively Affected Fiber Elongation
2.3. GhMYB201 Transcriptionally Activates Cell Wall Loosening-Related Genes
2.4. GhMYB201 Activates the Expression of GhKCSs and Changes the VLCFA Contents
3. Discussion
4. Materials and Methods
4.1. Identification and Cloning of GhMYB201s
4.2. Total RNA Isolation, qRT-PCR Analysis, and Transcriptome Analysis
4.3. Generation of Knockout Cottons
4.4. Observation of Cotton Fiber Phenotype
4.5. Arabidopsis Growth and Transformation
4.6. Transactivation Activity Assay in Yeast and Yeast One-Hybrid Assay
4.7. Transient Assays in Nicotiana Benthamiana
4.8. Fatty Acid Extractions
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Average Length of Upper Quartile Fibers (mm) | Length Uniformity (%) | Fiber Strength (cN/tex) | Micronaire Value | Fiber Elongation (%) | |
---|---|---|---|---|---|
WT | 29.5 ± 0.2 | 84.65 ± 0.95 | 31.6 ± 0.80 | 5.15 ± 0.05 | 6.40 ± 0.40 |
ghmyb201-38 | 21.25 ± 0.35 ** | 79.5 ± 0.20 * | 23.45 ± 1.15 * | 7.1 ± 0.10 ** | 6.10 ± 0.20 ns |
ghmyb201-45 | 22.25 ± 0.35 ** | 81.45 ± 0.05 ns | 23.5 ± 0.30 * | 7.25 ± 0.25 * | 6.40 ± 0.10 ns |
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Suo, Q.; Fang, N.; Zeng, J.; Yan, F.; Zhu, X.; Wang, Y.; Yu, W.; Chen, J.; Liang, A.; Li, Y.; et al. R2R3 MYB Transcription Factor GhMYB201 Promotes Cotton Fiber Elongation via Cell Wall Loosening and Very-Long-Chain Fatty Acid Synthesis. Int. J. Mol. Sci. 2024, 25, 9559. https://doi.org/10.3390/ijms25179559
Suo Q, Fang N, Zeng J, Yan F, Zhu X, Wang Y, Yu W, Chen J, Liang A, Li Y, et al. R2R3 MYB Transcription Factor GhMYB201 Promotes Cotton Fiber Elongation via Cell Wall Loosening and Very-Long-Chain Fatty Acid Synthesis. International Journal of Molecular Sciences. 2024; 25(17):9559. https://doi.org/10.3390/ijms25179559
Chicago/Turabian StyleSuo, Qingwei, Nianjuan Fang, Jianyan Zeng, Fulin Yan, Xi Zhu, Yi Wang, Wanting Yu, Junmin Chen, Aimin Liang, Yaohua Li, and et al. 2024. "R2R3 MYB Transcription Factor GhMYB201 Promotes Cotton Fiber Elongation via Cell Wall Loosening and Very-Long-Chain Fatty Acid Synthesis" International Journal of Molecular Sciences 25, no. 17: 9559. https://doi.org/10.3390/ijms25179559