Oxidative Stress and Annexin A2 Differential Expression in Free Fatty Acids-Induced Non-Alcoholic Fatty Liver Disease in HepG2 Cells
Abstract
:1. Introduction
2. Results
2.1. Oleic and Palmitic Acids Differentially Induce Lipid Accumulation in HepG2 Cells
2.2. Oleic and Palmitic Acids Impair Cellular Antioxidant Defense and Induce Oxidative Stress in HepG2 Cells
2.3. Oleic and Palmitic Acids Upregulate Transcriptional Expression of Inflammatory Cytokines
2.4. Oleic and Palmitic Acid Modulate KRT17 and ANXA2 Expression
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Cell Culture
4.3. FFA-Induced Steatosis and Cytotoxicity
4.4. Endogenous Antioxidant System
4.5. Oxidative Stress
4.6. qPCR Analysis
4.7. Western Blot
4.8. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primer | Sequence 5′-3′ | Amplicon (bp) |
---|---|---|
IL1β forward | CAGGATATGGAGCAACAAGTGG | 136 |
IL1β reverse | GGGCTTATCATCTTTCAACACGC | |
IL17 forward | CAATCCCACGAAATCCAGGATG | 156 |
IL17 reverse | GGTGGAGATTCCAAGGTGAGG | |
IL22 forward | CTGATAACAACACAGACGTTCG | 170 |
IL22 reverse | CCACCTCCTGCATATAAGGC | |
TGFβ forward | GTACCTGAACCCGTGTTGCTC | 107 |
TGFβ reverse | CAGGAATTGTTGCTGTATTTCTGG | |
IL6 forward | GATTCCAAAGATGTAGCCGCC | 242 |
IL6 reverse | ATTTTCACCAGGCAAGTCTCCTC | |
KRT17 forward | CTGATGACTTCCGCACCAAGTT | 234 |
KRT17 reverse | CAGCGTCCATCTCCACATTG | |
ANXA2 forward | TGACCAAGATGCTCGGGATC | 113 |
ANXA2 reverse | TTTCTGGAGGTGGGGCA | |
BM2 forward | AGCAGAGAATGGAAAGTCAAA | 94 |
BM2 reverse | TGTTGATGTTGGATAAGAGAA |
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Arruda, V.M.; Azevedo, G.T.; Granato, M.J.M.G.; Matos, A.C.P.; Araújo, T.G.; Guerra, J.F.d.C. Oxidative Stress and Annexin A2 Differential Expression in Free Fatty Acids-Induced Non-Alcoholic Fatty Liver Disease in HepG2 Cells. Int. J. Mol. Sci. 2024, 25, 9591. https://doi.org/10.3390/ijms25179591
Arruda VM, Azevedo GT, Granato MJMG, Matos ACP, Araújo TG, Guerra JFdC. Oxidative Stress and Annexin A2 Differential Expression in Free Fatty Acids-Induced Non-Alcoholic Fatty Liver Disease in HepG2 Cells. International Journal of Molecular Sciences. 2024; 25(17):9591. https://doi.org/10.3390/ijms25179591
Chicago/Turabian StyleArruda, Vinícius Marques, Gabriela Tolentino Azevedo, Maria Júlia Maia Gonçalves Granato, André Carlos Pereira Matos, Thaise Gonçalves Araújo, and Joyce Ferreira da Costa Guerra. 2024. "Oxidative Stress and Annexin A2 Differential Expression in Free Fatty Acids-Induced Non-Alcoholic Fatty Liver Disease in HepG2 Cells" International Journal of Molecular Sciences 25, no. 17: 9591. https://doi.org/10.3390/ijms25179591