Postmortem-Derived Exosomal MicroRNA 486-5p as Potential Biomarkers for Ischemic Heart Disease Diagnosis
Abstract
:1. Introduction
2. Results
2.1. Quality of Exosomal RNA Isolated from Postmortem Plasma and Pericardial Fluid
2.2. Analysis of Small RNA Profiling of Postmortem Exosomes Using Next-Generation Sequencing
2.3. Validation of Significantly Increased miRNA
2.4. miR-486-5p Expression in Advanced Atherosclerotic Lesions
3. Discussion
4. Materials and Methods
4.1. Sample Collection and Preparation
4.2. Information of Samples Used for Experiments
4.3. Exosomal RNA Isolation and Quality Control Analysis
4.4. Next-Generation Sequencing (NGS) Procedure
4.5. Validation Using Quantitative RT-PCR
4.6. Histopathology for Postmortem Human Coronary Artery
4.7. Statistical Analysis
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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miRNA ID | Control (log2) | Myocardial Infarction (log2) | Fold Change (H/CH) | p-Value | |
---|---|---|---|---|---|
Up | hsa-miR-3591-5p | 2.374 | 5.950 | 11.929 | 0.027 |
hsa-miR-874-3p | 3.845 | 5.575 | 3.319 | 0.024 | |
hsa-miR-423-5p | 10.870 | 12.405 | 2.897 | 0.012 | |
hsa-miR-486-5p | 13.363 | 14.698 | 2.522 | 0.045 | |
hsa-miR-3184-3p | 10.918 | 12.249 | 2.516 | 0.009 | |
Down | hsa-miR-4755-5p | 5.183 | 0.000 | 0.028 | 0.017 |
hsa-miR-554 | 4.762 | 0.000 | 0.037 | 0.018 | |
hsa-miR-4632-3p | 5.916 | 2.006 | 0.067 | 0.030 | |
hsa-miR-6729-3p | 6.337 | 2.540 | 0.072 | 0.033 | |
hsa-miR-1296-5p | 4.762 | 1.363 | 0.095 | 0.026 | |
hsa-miR-7107-5p | 5.586 | 2.483 | 0.116 | 0.005 | |
hsa-miR-3687 | 6.120 | 3.129 | 0.126 | 0.045 | |
hsa-miR-411-3p | 4.969 | 2.051 | 0.132 | 0.008 | |
hsa-miR-1237-3p | 4.762 | 2.006 | 0.148 | 0.043 | |
hsa-miR-6754-3p | 4.969 | 2.967 | 0.250 | 0.050 | |
hsa-miR-28-3p | 9.886 | 9.068 | 0.568 | 0.048 |
miRNA ID | Control (log2) | Myocardial Infarction (log2) | Fold Change (H/CH) | p-Value | |
---|---|---|---|---|---|
Up | hsa-miR-4443 | 8.389 | 10.849 | 5.503 | 0.009 |
hsa-miR-625-3p | 3.916 | 6.253 | 5.053 | 0.005 | |
Down | hsa-miR-1295a | 6.007 | 0.000 | 0.016 | 0.035 |
hsa-miR-4494 | 6.007 | 0.000 | 0.016 | 0.035 | |
hsa-miR-212-5p | 5.593 | 0.000 | 0.021 | 0.046 | |
hsa-miR-4781-3p | 7.629 | 2.830 | 0.036 | 0.026 | |
hsa-miR-379-5p | 7.035 | 2.354 | 0.039 | 0.029 | |
hsa-miR-5585-5p | 6.658 | 2.354 | 0.051 | 0.029 | |
hsa-miR-214-3p | 6.084 | 2.150 | 0.065 | 0.024 | |
hsa-miR-1287-5p | 7.808 | 3.899 | 0.067 | 0.039 | |
hsa-miR-5095 | 7.676 | 5.002 | 0.157 | 0.050 | |
hsa-miR-4497 | 11.467 | 8.905 | 0.169 | 0.041 | |
hsa-miR-1285-3p | 7.775 | 5.717 | 0.240 | 0.043 | |
hsa-miR-107 | 6.973 | 5.387 | 0.333 | 0.023 |
Control Group (n = 13) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
No. | Age | Sex | BMI (kg/m) | PMI | Cause of Death | Heart Weight (g) | Risk Factors | Luminal Stenosis (%) | Lesion Type | ||
DM | HTN | Smoking | |||||||||
1 | 52 | F | 22.2 | 26 h 10 m | Hanging | 300 | A + | A | A | 0 | I |
2 | 21 | F | 21.5 | 15 h 50 m | Sudden manhood death syndrome | 264 | A | A | A | 0 | I |
3 | 47 | F | 19.4 | 75 h 30 m | Drug intoxication | 276 | A | A | A | 56 | III |
4 | 70 | F | 23.4 | 42 h 10 m | Manual strangulation | 336 | A | A | A | 0 | I |
5 | 65 | M | 26.7 | 65 h 40 m | Drug intoxication | 460 | A | A | A | 83 | VII |
6 | 64 | M | 22.5 | 35 h 30 m | Drug intoxication | 372 | A | A | A | 83 | VI |
7 | 56 | F | 21.4 | 39 h 30 m | Hanging | 320 | A | A | A | 84 | VI |
8 | 58 | M | 16.5 | 53 h 50 m | Asphyxia | 304 | A | A | A | 66 | V |
9 | 50 | M | 28.1 | 87 h 00 m | Subarachnoid hemorrhage | 452 | A | A | A | 73 | IV |
10 | 74 | F | 19.9 | 65 h 30 m | Non-traumatic cerebral hemorrhage | 402 | A | A | A | 62 | IV |
11 | 54 | M | 16.2 | 45 h 30 m | Drug intoxication | 406 | A | A | A | 87 | VII |
12 | 25 | M | 27.4 | 86 h 00 m | Drug intoxication | 344 | A | A | A | 0 | I |
13 | 59 | F | 22.1 | 25 h 10 m | Drug intoxication | 372 | A | A | A | 0 | Ⅰ |
MI Group (n = 24) | |||||||||||
No. | Age | Sex | BMI (kg/m) | PMI | Cause of Death | Heart Weight (g) | Risk Factors | Luminal Stenosis (%) | Lesion Type | ||
DM | HTN | Smoking | |||||||||
1 | 66 | M | 29.3 | 31 h 50 m | Ischemic heart disease including acute myocardial infarction | 580 | A | P | A | 78 | VII |
2 | 52 | M | 31.7 | 39 h 20 m | 680 | A | A | A | 88 | V | |
3 | 54 | M | 26.2 | 21 h 40 m | 412 | A | A | A | 100 | VI * | |
4 | 55 | M | 26.7 | 24 h 20 m | 426 | A | A | A | 88 | V | |
5 | 49 | M | 24.8 | 48 h 55 m | 388 | A | A | A | 86 | V | |
6 | 59 | M | 22.4 | 37 h 50 m | 378 | A | A | A | 95 | V | |
7 | 35 | M | 21.4 | 48 h 15 m | 370 | A | A | A | 76 | VI | |
8 | 49 | M | 28.2 | 56 h 20 m | 434 | A | A | A | 95 | V | |
9 | 32 | M | 24.3 | 26 h 30 m | 422 | A | A | A | 96 | V | |
10 | 63 | M | 21 | 27 h 00 m | 444 | A | P | P | 75 | VII | |
11 | 55 | M | 24.8 | 42 h 05 m | 460 | A | A | A | 88 | VI | |
12 | 46 | M | 26.6 | 25 h 50 m | 404 | A | A | P | 84 | V | |
13 | 39 | M | 29.2 | 42 h 10 m | 530 | A | A | A | 94 | VI | |
14 | 76 | M | 15.3 | 39 h 00 m | 362 | A | A | A | 100 | V * | |
15 | 63 | M | 25.4 | 48 h 25 m | 520 | A | A | A | 85 | VI | |
16 | 55 | M | 25.1 | 97 h 05 m | 408 | A | A | A | 82 | VI | |
17 | 62 | M | 19.4 | 58 h 50 m | 400 | A | A | A | 87 | V | |
18 | 55 | M | 22.0 | 64 h 40 m | 422 | A | A | A | 89 | VI | |
19 | 52 | M | 27.4 | 26 h 30 m | 764 | A | A | A | 80 | V | |
20 | 57 | M | 25.5 | 67 h 50 m | 420 | A | A | A | 75 | VII | |
21 | 66 | M | 23.3 | 26 h 00 m | 610 | P | A | A | 85 | VII | |
22 | 59 | F | 18.9 | 97 h 45 m | 446 | A | A | A | 91 | VII | |
23 | 40 | M | 29.5 | 35 h 00 m | 462 | A | A | A | 92 | VII | |
24 | 44 | M | 22.5 | 29 h 00 m | 452 | A | A | A | 75 | VII |
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Kim, S.-Y.; Lee, S.; Park, J.-T.; Lee, S.-J.; Kim, H.-S. Postmortem-Derived Exosomal MicroRNA 486-5p as Potential Biomarkers for Ischemic Heart Disease Diagnosis. Int. J. Mol. Sci. 2024, 25, 9619. https://doi.org/10.3390/ijms25179619
Kim S-Y, Lee S, Park J-T, Lee S-J, Kim H-S. Postmortem-Derived Exosomal MicroRNA 486-5p as Potential Biomarkers for Ischemic Heart Disease Diagnosis. International Journal of Molecular Sciences. 2024; 25(17):9619. https://doi.org/10.3390/ijms25179619
Chicago/Turabian StyleKim, So-Yeon, Sookyoung Lee, Jong-Tae Park, Su-Jin Lee, and Hyung-Seok Kim. 2024. "Postmortem-Derived Exosomal MicroRNA 486-5p as Potential Biomarkers for Ischemic Heart Disease Diagnosis" International Journal of Molecular Sciences 25, no. 17: 9619. https://doi.org/10.3390/ijms25179619