Post-Transcriptional Induction of the Antiviral Host Factor GILT/IFI30 by Interferon Gamma
Abstract
:1. Introduction
2. Results
2.1. Post-Transcriptional Elevation of GILT Protein by IFN-γ in HeLa Cells
2.2. Post-Transcriptional Elevation of GILT Protein by IFN-γ in Other Cell Types
2.3. Absence of Activation of GILT Transcription by IFN-γ
2.4. GILT Protein Stability and Its Relationship with IFN-γ
2.5. Impact of Rapamycin on GILT Protein Induction by IFN-γ
2.6. Untranslated Region of GILT mRNA Inhibits Its Translation in the Absence of IFN-γ
2.7. STAT1 Phosphorylation Is Necessary for IFN-γ-Mediated GILT Protein Induction
3. Discussion
4. Materials and Methods
4.1. Plasmids
4.2. Cells
4.3. Peripheral Blood Mononuclear Cells
4.4. Amphotropic Murine Leukemia Virus Vector
4.5. Western Blotting
4.6. Luciferase Activity
4.7. Protein Stability
4.8. Droplet Digital PCR
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primers | Nucleotide Sequences |
---|---|
5′ UTR sense | CTGCAGTCGCCACACCTTGC |
GILT sense | ATGACTTCGAAAGTTTATGAT |
GILT antisense | CACTTGAAGCAAACACTCCTG |
5′ UTR-RLuc sense | CTGCAGTCGCCACACCTTTGCCCCTGCTG (5′ UTR of GILT) CG ATGACTTCGAAAGTTTATGAT (RLuc) |
RLuc antisense | TTATTGTTCATTTTTGAGAACTCGC |
3′ UTR sense | TGGCCGGTGAGCTGCGGAGAG |
3′ UTR antisense | GCTTATTAAACTAGTTTTACTTTAGC |
GAPDH sense | CCATGCCATCACTGCCACCC |
GAPDH antisense | GCCAGTGAGCTTCCCGTTCAG |
GILT 532 antisense | CTACAGGCATAGTGGCAGACT |
IFI6 sense | GCGCGCGGCGCCACCATGCGG |
IFI6 antisense | TGGCTACTCCTCATCCTCCTC |
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Nakamura, T.; Izumida, M.; Hans, M.B.; Suzuki, S.; Takahashi, K.; Hayashi, H.; Ariyoshi, K.; Kubo, Y. Post-Transcriptional Induction of the Antiviral Host Factor GILT/IFI30 by Interferon Gamma. Int. J. Mol. Sci. 2024, 25, 9663. https://doi.org/10.3390/ijms25179663
Nakamura T, Izumida M, Hans MB, Suzuki S, Takahashi K, Hayashi H, Ariyoshi K, Kubo Y. Post-Transcriptional Induction of the Antiviral Host Factor GILT/IFI30 by Interferon Gamma. International Journal of Molecular Sciences. 2024; 25(17):9663. https://doi.org/10.3390/ijms25179663
Chicago/Turabian StyleNakamura, Taisuke, Mai Izumida, Manya Bakatumana Hans, Shuichi Suzuki, Kensuke Takahashi, Hideki Hayashi, Koya Ariyoshi, and Yoshinao Kubo. 2024. "Post-Transcriptional Induction of the Antiviral Host Factor GILT/IFI30 by Interferon Gamma" International Journal of Molecular Sciences 25, no. 17: 9663. https://doi.org/10.3390/ijms25179663