Anti-Inflammatory and Anti-(Lymph)angiogenic Properties of an ABCB5+ Limbal Mesenchymal Stem Cell Population
Abstract
:1. Introduction
2. Results
2.1. ABCB5 Is Expressed in the Human Limbal/Corneal Stroma
2.2. FACS-Sorted ABCB5+ Limbal Fibroblastic Cells Exhibit MSC Characteristics
2.3. ABCB5+ Cells Exhibit Higher Migratory Capability in the Presence of SDF1
2.4. The Paracrine Activity of ABCB5+ Cells Reduces the Metabolic Activity of Blood and Lymphatic Endothelial Cells and Suppresses the Proliferation of Jurkat Cells
2.5. The ABCB5+ Limbal Stromal Cells Produced Lower Levels of the Pro-(Lymph)angiogenic Regulators VEGFA, VEGFC, and the Pro-Inflammatory Cytokine MIF
3. Discussion
4. Materials and Methods
4.1. Ethics Statement for the Use of Human Tissue
4.2. Primary Human Limbal Stromal Cell Harvesting and Maintenance
4.3. Maintenance of Human Lymphatic and Blood Endothelial Cells
4.4. FACS Sorting
4.5. FACS Analysis
4.6. Mesenchymal Stem Cell Phenotypic Characterisation
4.6.1. Colony-Formation Units-Fibroblast Assay
4.6.2. Trilineage Differentiation Protocols
4.6.3. Oil Red O Staining
4.6.4. Alcian Blue Staining
4.6.5. Alizarin Red Staining
4.7. Immunohistochemistry
4.8. Collection of Conditioned Media
4.9. Paracrine Activity of ABCB5+ vs. ABCB5- Cells on LECs and BECs
4.9.1. Alamar Blue Assay
4.9.2. Tube-Formation Assay
4.10. Co-Culture of Limbal Stromal Cells with Jurkat Cells and Measurement of Proliferation, Cell Cycle
4.11. Cell Migration Assay
4.12. Cytokine Asessment in ABCB5+ and ABCB5- Secretome
4.12.1. Protein Array
4.12.2. ELISA
4.13. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Meshko, B.; Volatier, T.L.A.; Mann, J.; Kluth, M.A.; Ganss, C.; Frank, M.H.; Frank, N.Y.; Ksander, B.R.; Cursiefen, C.; Notara, M. Anti-Inflammatory and Anti-(Lymph)angiogenic Properties of an ABCB5+ Limbal Mesenchymal Stem Cell Population. Int. J. Mol. Sci. 2024, 25, 9702. https://doi.org/10.3390/ijms25179702
Meshko B, Volatier TLA, Mann J, Kluth MA, Ganss C, Frank MH, Frank NY, Ksander BR, Cursiefen C, Notara M. Anti-Inflammatory and Anti-(Lymph)angiogenic Properties of an ABCB5+ Limbal Mesenchymal Stem Cell Population. International Journal of Molecular Sciences. 2024; 25(17):9702. https://doi.org/10.3390/ijms25179702
Chicago/Turabian StyleMeshko, Berbang, Thomas L. A. Volatier, Johanna Mann, Mark A. Kluth, Christoph Ganss, Markus H. Frank, Natasha Y. Frank, Bruce R. Ksander, Claus Cursiefen, and Maria Notara. 2024. "Anti-Inflammatory and Anti-(Lymph)angiogenic Properties of an ABCB5+ Limbal Mesenchymal Stem Cell Population" International Journal of Molecular Sciences 25, no. 17: 9702. https://doi.org/10.3390/ijms25179702